20.500.12556/DiRROS-19487 Virome analysis of signal crayfish (Pacifastacus leniusculus) along its invasion range reveals diverse and divergent RNA viruses Crayfish are a keystone species of freshwater ecosystems and a successful invasive species. However, their pathogens, including viruses, remain understudied. The aim of this study was to analyze the virome of the invasive signal crayfish (Pacifastacus leniusculus) and to elucidate the potential differences in viral composition and abundance along its invasion range in the Korana River, Croatia. By the high-throughput sequencing of ribosomal RNA, depleted total RNA isolated from the crayfish hepatopancreas, and subsequent sequence data analysis, we identified novel and divergent RNA viruses, including signal crayfish-associated reo-like, hepe-like, toti-like, and picorna-like viruses, phylogenetically related to viruses previously associated with crustacean hosts. The patterns of reads abundance and calculated nucleotide diversities of the detected viral sequences varied along the invasion range. This could indicate the possible influence of different factors and processes on signal crayfish virome composition: e.g., the differences in signal crayfish population density, the non-random dispersal of host individuals from the core to the invasion fronts, and the transfer of viruses from the native co-occurring and phylogenetically related crayfish species. The study reveals a high, previously undiscovered diversity of divergent RNA viruses associated with signal crayfish, and sets foundations for understanding the potential risk of virus transmissions as a result of this invader’s dispersal. signal crayfish virome RNA viruses invasive alien species invasion range high-throughput sequencing true false true Angleški jezik Ni določen Neznano 2024-07-18 03:52:40 2024-07-19 11:51:19 2024-07-20 04:03:04 0000-00-00 00:00:00 2021 0 0 Soavtorji: Denis Kutnjak , Silvija Černi, Ana Bielen, Sandra Hudina; Nasl. z nasl. zaslona; Opis vira z dne 17. 11. 2021; Št. članka: 2259; str. 1-21 iss. 11 Vol. 13 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-11-11 579 1999-4915 10.3390/v13112259 85287171 https://www.mdpi.com/1999-4915/13/11/2259/htm 1 aa200c09-4497-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26888 RAZ_Bacnik_Katarina_2021.pdf RAZ_Bacnik_Katarina_2021.pdf 1 004CDC363EAC0825AE731B7C5E9A5277 a7acb2fb2ab16b7aca2a2044648b595c94dfd0064410239d62d445f02ab1bb04 dc2fa9b6-45a3-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26994 Nacionalni inštitut za biologijo 0 0 0