20.500.12556/DiRROS-19474 Inactivation of pepper mild mottle virus in water by cold atmospheric plasma Water scarcity is one of the greatest threats for human survival and quality of life, and this is increasingly contributing to the risk of human, animal and plant infections due to waterborne viruses. Viruses are transmitted through polluted water, where they can survive and cause infections even at low concentrations. Plant viruses from the genus Tobamovirus are highly mechanically transmissible, and cause considerable damage to important crops, such as tomato. The release of infective tobamoviruses into environmental waters has been reported, with the consequent risk for arid regions, where these waters are used for irrigation. Virus inactivation in water is thus very important and cold atmospheric plasma (CAP) is emerging in this field as an efficient, safe, and sustainable alternative to classic waterborne virus inactivation methods. In the present study we evaluated CAP-mediated inactivation of pepper mild mottle virus (PMMoV) in water samples. PMMoV is a very resilient water-transmissible tobamovirus that can survive transit through the human digestive tract. The efficiency of PMMoV inactivation was characterized for infectivity and virion integrity, and at the genome level, using test plant infectivity assays, transmission electron microscopy, and molecular methods, respectively. Additionally, the safety of CAP treatment was determined by testing the cytotoxic and genotoxic properties of CAP-treated water on the HepG2 cell line. 5-min treatment with CAP was sufficient to inactivate PMMoV without introducing any cytotoxic or genotoxic effects in the in-vitro cell model system. These data on inactivation of such stable waterborne virus, PMMoV, will encourage further examination of CAP as an alternative for treatment of potable and irrigation waters, and even for other water sources, with emphasis on inactivation of various viruses including enteric viruses. enteric viruses pepper mild mottle virus virus inactivation water decontamination cold atmospheric plasma true false true Angleški jezik Ni določen Neznano 2024-07-18 03:51:55 2024-07-19 09:50:24 2024-07-20 04:03:03 0000-00-00 00:00:00 2021 0 0 Nasl. z nasl. zaslona; Opis vira z dne 2. 2. 2021; str. 1-12 Vol. 12 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-01-28 578 1664-302X 10.3389/fmicb.2021.618209 49725955 https://www.frontiersin.org/articles/10.3389/fmicb.2021.618209/full 1 8f8f2f6e-4497-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26875 RAZ_Filipic_Arijana_2021.pdf RAZ_Filipic_Arijana_2021.pdf 1 EFCD6DE568A5114FF089429DAC3DE6AE 072f3d56fde0ac6f01facfa18f996146c2b950985a1c26e796fa8b23868dff8a 2bbe680b-4594-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26966 Nacionalni inštitut za biologijo 0 0 0