20.500.12556/DiRROS-19469 Polysaccharide-based bilayer coatings for biofilm-inhibiting surfaces of medical devices Chitosan (Chi) and 77KS, a lysine-derived surfactant, form polyelectrolyte complexes that reverse their charge from positive to negative at higher 77KS concentrations, forming aggregates that have been embedded with amoxicillin (AMOX). Dispersion of this complex was used to coat polydimethylsiloxane (PDMS) films, with an additional layer of anionic and hydrophilic hyaluronic acid (HA) as an outer adsorbate layer to enhance protein repulsion in addition to antimicrobial activity by forming a highly hydrated layer in combination with steric hindrance. The formed polysaccharide-based bilayer on PDMS was analyzed by water contact angle measurements, X-ray photoelectron spectroscopy (XPS), and surface zeta (ζ)-potential. All measurements show the existence and adhesion of the two layers on the PDMS surface. Part of this study was devoted to understanding the underlying protein adsorption phenomena and identifying the mechanisms associated with biofouling. Thus, the adsorption of a mixed-protein solution (bovine serum albumin, fibrinogen, γ-globulin) on PDMS surfaces was studied to test the antifouling properties. The adsorption experiments were performed using a quartz crystal microbalance with dissipation monitoring (QCM-D) and showed improved antifouling properties by these polysaccharide-based bilayer coatings compared to a reference or for only one layer, i.e., the complex. This proves the benefit of a second hyaluronic acid layer. Microbiological and biocompatibility tests were also performed on real samples, i.e., silicone discs, showing the perspective of the prepared bilayer coating for medical devices such as prostheses, catheters (balloon angioplasty, intravascular), delivery systems (sheaths, implants), and stents. polysaccharides chitosan hyaluronic acid anti-biofilm silicone polisaharidi hitozan hialuronska kislina anti-biofilmska prevleka silikoni true false true Angleški jezik Slovenski jezik Neznano 2024-07-18 03:51:34 2024-07-19 11:13:39 2024-07-20 04:03:01 0000-00-00 00:00:00 2021 0 0 Soavtorji: Thomas Luxbacher, Alenka Vesel, Alja Štern, Bojana Žegura, Janja Trček, Lidija Fras Zemljič; Nasl. z nasl. zaslona; Opis vira z dne 25. 8. 2021; Št. članka 720; str. 1-22 iss. 16 Vol. 14 Aug. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-08-21 677.017:544.7 1996-1944 10.3390/ma14164720 73991171 https://www.mdpi.com/1996-1944/14/16/4720 1 82b2f945-4497-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26870 RAZ_Ajdnik_Urban_2021.pdf RAZ_Ajdnik_Urban_2021.pdf 1 3185A94C8888F3821D4F3E9672E8EDE5 898387eb7fd04c666d03c488e41acf1b5ab4cd5de4defd9d546d48991f49a72c 81460df4-459e-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26984 Nacionalni inštitut za biologijo 0 0 0