20.500.12556/DiRROS-19466 Prunus persica plant endogenous peptides PpPep1 and PpPep2 cause PTI-like transcriptome reprogramming in peach and enhance resistance to Xanthomonas arboricola pv. pruni Background Rosaceae species are economically highly relevant crops. Their cultivation systems are constrained by phytopathogens causing severe losses. Plants respond to invading pathogens through signaling mechanisms, a component of which are of them being plant elicitor peptides (Peps). Exogenous application of Peps activates defense mechanisms and reduces the symptoms of pathogen infection in various pathosystems. We have previously identified the Rosaceae Peps and showed, in an ex vivo system, that their topical application efficiently enhanced resistance to the bacterial pathogen Xanthomonas arboricola pv. pruni (Xap). Results Here we demonstrate the effectiveness of Prunus persica peptides PpPep1 and PpPep2 in protecting peach plants in vivo at nanomolar doses, with 40% reduction of the symptoms following Xap massive infection. We used deep sequencing to characterize the transcriptomic response of peach plants to preventive treatment with PpPep1 and PpPep2. The two peptides induced highly similar massive transcriptomic reprogramming in the plant. One hour, 1 day and 2 days after peptide application there were changes in expression in up to 8% of peach genes. We visualized the transcriptomics dynamics in a background knowledge network and detected the minor variations between plant responses to PpPep1 and PpPep2, which might explain their slightly different protective effects. By designing a P. persica Pep background knowledge network, comparison of our data and previously published immune response datasets was possible. Conclusions Topical application of P. persica Peps mimics the PTI natural response and protects plants against massive Xap infection. This makes them good candidates for deployment of natural, targeted and environmental-friendly strategies to enhance resistance in Prunus species and prevent important biotic diseases. obramba rastlin rastlinski elicitorni peptidi (Pep) zaporedje RNA genomi true false true Angleški jezik Slovenski jezik Neznano 2024-07-18 03:51:25 2024-07-19 10:21:06 2024-07-20 04:03:01 0000-00-00 00:00:00 2021 0 0 Nasl. z nasl. zaslona; Opis vira z dne 20. 5. 2021; str. 1-18 Vol. 22 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-05-18 60 1471-2164 10.1186/s12864-021-07571-9 64029443 https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/s12864-021-07571-9.pdf 1 7d6c8d88-4497-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26867 RAZ_Foix_Laura_2021.pdf RAZ_Foix_Laura_2021.pdf 1 BC56681F9DE36F5AADECC33AA19909E0 53979e7b0b8bdfe987a918079aefc58197e4f62d7ff915a632b7bd83fa9aa930 b3e9f43b-4597-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26972 Nacionalni inštitut za biologijo 0 0 0