20.500.12556/DiRROS-19454 Metagenomic characterization of parental and production CHO cell lines for detection of adventitious viruses Viral contamination is a major concern for biological products. Therefore, virus testing of raw materials and cells is essential for the safety of the final product. We used high-throughput sequencing to detect viral-like sequences in selected CHO cell lines. Our aim was to test various approaches of sample preparation, to establish a pipeline for metagenomic analysis and to characterize standard viral metagenome of production and parental CHO cell lines. The comparison of the metagenomics composition of the differently prepared samples showed that among four tested approaches sequencing of ribosomal RNA depleted total RNA is the most promising approach. The metagenomics investigation of one production and three parental CHO cell lines of diverse origin did not indicate the presence of adventitious viral agents in the investigated samples. The study revealed an expected background of virus-like nucleic acids in the samples, which originate from remains of expression vectors, endogenized viral elements and residuals of bacteriophages. virologija metagenomija koronavirus odpadne vode true false true Angleški jezik Slovenski jezik Neznano 2024-07-18 03:50:34 2024-07-19 09:07:53 2024-07-20 04:03:00 0000-00-00 00:00:00 2021 0 0 Soavtorji: Denis Kutnjak Barbara Jerič Kokelj, Nika Tuta, Tan Lončar, Matjaž Vogelsang, Maja Ravnikar; str. 70-75 Vol. 69 Jan. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-01-01 578 1045-1056 10.1016/j.biologicals.2020.11.001 39971587 https://www.sciencedirect.com/science/article/pii/S1045105620301354 1 5f561ded-4497-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26855 RAZ_Bacnik_Katarina_2021.pdf RAZ_Bacnik_Katarina_2021.pdf 1 1E7522FC59B66BA9B650CE6B4B248E75 40156c08400590213857d1bbcc9e7465d667605c8995a783098162ab1f47ad6c 11d0847d-458d-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26961 Nacionalni inštitut za biologijo 0 0 0