20.500.12556/DiRROS-19369 Development and validation of a one-step reverse transcription real-time PCR assay for simultaneous detection and identification of tomato mottle mosaic virus and tomato brown rugose fruit virus Tobamovirus species represent a threat to solanaceous crops worldwide, due to their extreme stability and because they are seed borne. In particular, recent outbreaks of tomato brown rugose fruit virus in tomato and pepper crops led to the establishment of prompt control measures, and the need for reliable diagnosis was urged. Another member of the genus, tomato mottle mosaic virus, has recently gained attention due to reports in different continents and its common features with tomato brown rugose fruit virus. In this study, a new real-time RT-PCR detection system was developed for tomato brown rugose fruit virus and tomato mottle mosaic virus on tomato leaves and seeds using TaqMan chemistry. This test was designed to detect tomato mottle mosaic virus by amplifying the movement protein gene in a duplex assay with the tomato brown rugose fruit virus target on the CP-3’NTR region, which was previously validated as a single assay. The performance of this test was evaluated, displaying analytical sensitivity 10−5–10−6-fold dilution for seeds and leaves, respectively, and good analytical specificity, repeatability, and reproducibility. Using the newly developed and validated test, tomato brown rugose fruit virus detection was 100% concordant with previously performed analyses on 106 official samples collected in 2021 from different continents. real-time PCR tomato mottle mosaic virus tomato brown rugose fruit virus leaves detection seeds detections performance criteria Solanum lycopersicum Capsicum annum biologija rastline paradižnik virusi true false true Angleški jezik Latinski jezik Neznano 2024-07-16 16:26:25 2024-07-16 16:26:25 2024-07-17 03:59:15 0000-00-00 00:00:00 2022 0 0 Soavtorji: Ariana Manglli, Anna Taglienti, Ana Vučurović,Jakob Brodarič, Luca Ferretti, Marta Luigi, Andrea Gentili, Nataša Mehle; Nasl. z nasl. zaslona; Opis vira z dne 14. 2. 2022; Št. članka: 489; str. 1-20 iss. 4 Vol. 11 11 Feb. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-02-11 632 2223-7747 10.3390/plants11040489 97172483 https://www.mdpi.com/2223-7747/11/4/489 1 a0ada9f9-436e-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26715 RAZ_Tiberini_Antonio_2022.pdf RAZ_Tiberini_Antonio_2022.pdf 1 35CB1DAA2F957D99ABEB5EFF3F46EC9B 560a3d20afb72ddcfaf79c1c02f4b7f677c7446a7e0975df6d9358e269390c39 223e77c9-436f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26716 Nacionalni inštitut za biologijo 0 0 0