20.500.12556/DiRROS-19365 Nanoscale transformations of amphiboles within human alveolar epithelial cells Amphibole asbestos is related to lung fibrosis and several types of lung tumors. The disease-triggering mechanisms still challenge our diagnostic capabilities and are still far from being fully understood. The literature focuses primarily on the role and formation of asbestos bodies in lung tissues, but there is a distinct lack of studies on amphibole particles that have been internalized by alveolar epithelial cells (AECs). These internalized particles may directly interact with the cell nucleus and the organelles, exerting a synergistic action with asbestos bodies (AB) from a different location. Here we document the near-atomic- to nano-scale transformations induced by, and taking place within, AECs of three distinct amphiboles (anthophyllite, grunerite, “amosite”) with different Fe-content and morphologic features. We show that: (i) an Fe-rich layer is formed on the internalized particles, (ii) particle grain boundaries are transformed abiotically by the internal chemical environment of AECs and/or by a biologically induced mineralization mechanism, (iii) the Fe-rich material produced on the particle surface does not contain large amounts of P, in stark contrast to extracellular ABs, and (iv) the iron in the Fe-rich layer is derived from the particle itself. Internalized particles and ABs follow two distinct formation mechanisms reaching different physicochemical end-states. amphiboles human alveolar epithelial cells asbestos lung cancer nanoscale investigation acS/TEM-EDXS dual-EELS asbestos-related tumors materiali azbest bolezni zdravje pljučna fibroza true false true Angleški jezik Slovenski jezik Neznano 2024-07-16 12:26:13 2024-07-16 12:26:13 2024-07-17 03:59:15 0000-00-00 00:00:00 2022 0 0 Soavtorji: Maja Jamnik, Goran Dražić, Marjetka Podobnik, Magda Tušek Žnidarič, Giancarlo Della Ventura, Günther J. Redhammer, Nada Žnidaršič, Simon Caserman & Reto Gieré; Nasl. z nasl. zaslona; Opis vira z dne 9. 2. 2022; Št. članka: 1782; str. 1-14 Vol. 12 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-02-02 620.1/.2 2045-2322 10.1038/s41598-022-05802-x 96630275 https://www.nature.com/articles/s41598-022-05802-x 1 11fc0547-434d-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26706 RAZ_Vigliaturo_Ruggero_2022.pdf RAZ_Vigliaturo_Ruggero_2022.pdf 1 2D177548C6517CBCD767E80AC514D728 ec3b013f9eb78459d973842aeefc70a398f4c762dec55d138850582d1cf469fe d60e7277-434d-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26707 Nacionalni inštitut za biologijo 0 0 0