20.500.12556/DiRROS-19349 Do cytotoxicity and cell death cause false positive results in the in vitro comet assay? The comet assay is used to measure DNA damage induced by chemical and physical agents. High concentrations of test agents may cause cytotoxicity or cell death, which may give rise to false positive results in the comet assay. Systematic studies on genotoxins and cytotoxins (i.e. non-genotoxic poisons) have attempted to establish a threshold of cytotoxicity or cell death by which DNA damage results measured by the comet assay could be regarded as a false positive result. Thresholds of cytotoxicity/cell death range from 20% to 50% in various publications. Curiously, a survey of the latest literature on comet assay results from cell culture studies suggests that one-third of publications did not assess cytotoxicity or cell death. We recommend that it should be mandatory to include results from at least one type of assay on cytotoxicity, cell death or cell proliferation in publications on comet assay results. A combination of cytotoxicity (or cell death) and proliferation (or colony forming efficiency assay) is preferable in actively proliferating cells because it covers more mechanisms of action. Applying a general threshold of cytotoxicity/cell death to all types of agents may not be applicable; however, 25% compared to the concurrent negative control seems to be a good starting value to avoid false positive comet assay results. Further research is needed to establish a threshold value to distinguish between true and potentially false positive genotoxic effects detected by the comet assay. comet assay cytotoxicity genotoxicity DNA damage cell death test komet citotoksičnost genotoksičnost poškodbe DNK celična smrt true false true Angleški jezik Slovenski jezik Neznano 2024-07-16 03:44:50 2024-07-17 09:26:12 2024-07-18 03:53:59 0000-00-00 00:00:00 2022 0 0 Ostali avtorji: Helga Stopper, Bojana Zegura, Maria Dusinska, Peter Møller; Članek v PDF formatu obsega 9 str.; First online: 23 June 2022; str. 1-9 Vol. 881 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-09-01 576 1383-5718 10.1016/j.mrgentox.2022.503520 113873411 https://doi.org/10.1016/j.mrgentox.2022.503520 1 febf7f24-4314-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26673 RAZ_Azqueta_Amaya_2022.pdf RAZ_Azqueta_Amaya_2022.pdf 1 47D99F39CDB17A4AF889DCDC499EE3E5 575bd31c58aa7804b0813fbe7b5c2aa6abf5e700252782a5c01329276a46d0ab bbfe14fb-43fd-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26813 Nacionalni inštitut za biologijo 0 0 0