20.500.12556/DiRROS-19342 Fermentative indole production via bacterial tryptophan synthase alpha subunit and plant indole-3-glycerol phosphate lyase enzymes Indole is produced in nature by diverse organisms and exhibits a characteristic odor described as animal, fecal, and floral. In addition, it contributes to the flavor in foods, and it is applied in the fragrance and flavor industry. In nature, indole is synthesized either from tryptophan by bacterial tryptophanases (TNAs) or from indole-3-glycerol phosphate (IGP) by plant indole-3-glycerol phosphate lyases (IGLs). While it is widely accepted that the tryptophan synthase α-subunit (TSA) has intrinsically low IGL activity in the absence of the tryptophan synthase β-subunit, in this study, we show that Corynebacterium glutamicum TSA functions as a bona fide IGL and can support fermentative indole production in strains providing IGP. By bioprospecting additional bacterial TSAs and plant IGLs that function as bona fide IGLs were identified. Capturing indole in an overlay enabled indole production to titers of about 0.7 g L–1 in fermentations using C. glutamicum strains expressing either the endogenous TSA gene or the IGL gene from wheat. Corynebacterium glutamicum indole indole-3-glycerol phosphate lyase tryptophan synthase α-subunit bioprospecting fermentative production indol genetika biotehnologija rastlin true false true Angleški jezik Slovenski jezik Neznano 2024-07-16 03:44:05 2024-07-16 17:38:02 2024-07-17 03:59:13 0000-00-00 00:00:00 2022 0 0 str. 5634-5645 no. 18 Vol. 70 2. May 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-05-02 60:632 0021-8561 10.1021/acs.jafc.2c01042 107216387 https://pubs.acs.org/doi/10.1021/acs.jafc.2c01042 1 e489f771-4314-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26666 RAZ_Ferrer_Lenny_2022.pdf RAZ_Ferrer_Lenny_2022.pdf 1 A6FE1B294683FAD26A9E286D6DE976B8 c4711e5dad2b7fc7512688ac94b58ba045d4db6e48eba4c1d05f385cc56c9ede 1cebc639-4379-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26732 Nacionalni inštitut za biologijo 0 0 0