20.500.12556/DiRROS-19329 Bacterial indicators are ubiquitous members of pelagic microbiome in anthropogenically impacted coastal ecosystem Coastal zones are exposed to various anthropogenic impacts, such as different types of wastewater pollution, e.g., treated wastewater discharges, leakage from sewage systems, and agricultural and urban runoff. These various inputs can introduce allochthonous organic matter and microbes, including pathogens, into the coastal marine environment. The presence of fecal bacterial indicators in the coastal environment is usually monitored using traditional culture-based methods that, however, fail to detect their uncultured representatives. We have conducted a year-around in situ survey of the pelagic microbiome of the dynamic coastal ecosystem, subjected to different anthropogenic pressures to depict the seasonal and spatial dynamics of traditional and alternative fecal bacterial indicators. To provide an insight into the environmental conditions under which bacterial indicators thrive, a suite of environmental factors and bacterial community dynamics were analyzed concurrently. Analyses of 16S rRNA amplicon sequences revealed that the coastal microbiome was primarily structured by seasonal changes regardless of the distance from the wastewater pollution sources. On the other hand, fecal bacterial indicators were not affected by seasons and accounted for up to 34% of the sequence proportion for a given sample. Even more so, traditional fecal indicator bacteria (Enterobacteriaceae) and alternative wastewater-associated bacteria (Lachnospiraceae, Ruminococcaceae, Arcobacteraceae, Pseudomonadaceae and Vibrionaceae) were part of the core coastal microbiome, i.e., present at all sampling stations. Microbial source tracking and Lagrangian particle tracking, which we employed to assess the potential pollution source, revealed the importance of riverine water as a vector for transmission of allochthonous microbes into the marine system. Further phylogenetic analysis showed that the Arcobacteraceae in our data set was affiliated with the pathogenic Arcobacter cryaerophilus, suggesting that a potential exposure risk for bacterial pathogens in anthropogenically impacted coastal zones remains. We emphasize that molecular analyses combined with statistical and oceanographic models may provide new insights for environmental health assessment and reveal the potential source and presence of microbial indicators, which are otherwise overlooked by a cultivation approach. bakterije pelagial ekologija morja true false true Angleški jezik Slovenski jezik Neznano 2024-07-16 03:42:44 2024-07-16 05:26:11 2024-07-17 03:59:12 0000-00-00 00:00:00 2022 0 0 Nasl. z nasl. zaslona; Opis vira z dne 19. 1. 2022; Eduard Fadeev, Katja Klun, Matjaž Ličer, Tinkara Tinta, Valentina Turk; Št. članka: 765091; str. 1-21 Vol. 12 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-01-17 574 1664-302X 10.3389/fmicb.2021.765091 94163459 https://www.frontiersin.org/articles/10.3389/fmicb.2021.765091/full 1 b3e2b10d-4314-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26653 RAZ_Orel_Neza_2022.pdf RAZ_Orel_Neza_2022.pdf 1 5D04C56E4579DFC32D836CA02D68902B 9fe5149a1634665aa34a4692694b55ebf589e4b359b68d44cb2bff3f14596eb0 90fc59f2-4325-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26684 Nacionalni inštitut za biologijo 0 0 0