20.500.12556/DiRROS-19327 Biological and genetic characterization of Physostegia chlorotic mottle virus in Europe based on host range, location, and time Application of high throughput sequencing (HTS) technologies enabled the first identification of Physostegia chlorotic mottle virus (PhCMoV) in 2018 in Austria. Subsequently, PhCMoV was detected in Germany and Serbia on tomatoes showing severe fruit mottling and ripening anomalies. We report here how prepublication data-sharing resulted in an international collaboration across eight laboratories in five countries, enabling an in-depth characterization of PhCMoV. The independent studies converged toward its recent identification in eight additional European countries and confirmed its presence in samples collected 20 years ago (2002). The natural plant host range was expanded from two to nine species across seven families, and we confirmed the association of PhCMoV presence with severe fruit symptoms on economically important crops such as tomato, eggplant, and cucumber. Mechanical inoculations of selected isolates in the greenhouse established the causality of the symptoms on a new indexing host range. In addition, phylogenetic analysis showed a low genomic variation across the 29 near-complete genome sequences available. Furthermore, a strong selection pressure within a specific ecosystem was suggested by nearly identical sequences recovered from different host plants through time. Overall, this study describes the European distribution of PhCMoV on multiple plant hosts, including economically important crops on which the virus can cause severe fruit symptoms. This work demonstrates how to efficiently improve knowledge on an emergent pathogen by sharing HTS data and provides a solid knowledge foundation for further studies on plant rhabdoviruses. biološka karakterizacija genetska karakterizacija PhCMoV rastlinski virusi true false true Angleški jezik Slovenski jezik Neznano 2024-07-16 03:42:26 2024-07-17 09:58:44 2024-07-18 03:53:58 0000-00-00 00:00:00 2022 0 0 Ostali avtorji: Arnaud G. Blouin, Kris De Jonghe, Yoika Foucart, Marleen Botermans, Marcel Westenberg, Ruben Schoen, Pascal Gentit, Michèle Visage, Eric Verdin, Catherine Wipf-Scheibel, Heiko Ziebell, Yahya Z. A. Gaafar, Amjad Zia, Xiao-Hua Yan, Katja R. Richert-Pöggeler, Roswitha Ulrich, Mark Paul S. Rivarez, Denis Kutnjak, Ana Vučurović, and Sébastien Massart; str. 2797-2807 no. 11 Vol. 106 Nov. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-10-12 578 0191-2917 10.1094/PDIS-12-21-2800-RE 118755331 https://doi.org/10.1094/PDIS-12-21-2800-RE 1 a9071003-4314-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26651 RAZ_Temple_Coline_2022.pdf RAZ_Temple_Coline_2022.pdf 1 248D58FFFED462BA934766B403F25523 cb08b1807e258662a5ff36e0e228f3b0a9e021add3d83e3cf5a3c436723a649b 1f31c29e-4402-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26817 Nacionalni inštitut za biologijo 0 0 0