20.500.12556/DiRROS-19302 Cell-type proteomic and metabolomic resolution of early and late grain filling stages of wheat endosperm The nutritional value of wheat grains, particularly their protein and metabolite composition, is a result of the grain-filling process, especially in the endosperm. Here, we employ laser microdissection (LMD) combined with shotgun proteomics and metabolomics to generate a cell type-specific proteome and metabolome inventory of developing wheat endosperm at the early (15 DAA) and late (26 DAA) grain-filling stages. We identified 1803 proteins and 41 metabolites from four different cell types (aleurone (AL), sub-aleurone (SA), starchy endosperm (SE) and endosperm transfer cells (ETCs). Differentially expressed proteins were detected, 67 in the AL, 31 in the SA, 27 in the SE and 50 in the ETCs between these two-time points. Cell-type accumulation of specific SUT and GLUT transporters, sucrose converting and starch biosynthesis enzymes correlate well with the respective sugar metabolites, suggesting sugar upload and starch accumulation via nucellar projection and ETC at 15 DAA in contrast to the later stage at 26 DAA. Changes in various protein levels between AL, SA and ETC support this metabolic switch from 15 to 26 DAA. The distinct spatial and temporal abundances of proteins and metabolites revealed a contrasting activity of nitrogen assimilation pathways, e.g. for GOGAT, GDH and glutamic acid, in the different cell types from 15 to 26 DAA, which can be correlated with specific protein accumulation in the endosperm. The integration of cell-type specific proteome and metabolome data revealed a complex metabolic interplay of the different cell types and a functional switch during grain development and grain-filling processes. wheat proteomics and metabolomics aleurone sub-aleurone starchy endosperm endosperm transfer cells pšenični endosperm proteomska ločljivost metabolomska ločljivost true false true Angleški jezik Slovenski jezik Neznano 2024-07-15 09:19:04 2024-07-15 09:19:04 2024-07-16 03:45:12 0000-00-00 00:00:00 2023 0 0 Slovenski soavtorici: Živa Ramšak, Kristina Gruden; First online: 4 December 2023; str. 1-17 iss. Vol. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-03-01 577 1467-7644 10.1111/pbi.14203 177668867 https://doi.org/10.1111/pbi.14203 1 8639cec9-427a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26618 RAZ_Zhang_Shuang_2023.pdf RAZ_Zhang_Shuang_2023.pdf 1 A06E80C8FACBE7596CB2E9E3C94C2F2F 9f57bbdc843e499c9fa908cb6d7e6fe954db43dfbde20d296ef358cc9f6be6ca 8fc1ab12-427b-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26619 Nacionalni inštitut za biologijo 0 0 0