20.500.12556/DiRROS-19281 Significance of nuclear factor - kappa beta activation on prostate needle biopsy samples in the evaluation of Gleason score 6 prostatic carcinoma indolence The goal of our study was to find out whether the immunohistochemical expression of nuclear factor-kappa beta (NF-%B) p65 in biopsy samples with Gleason score 3 + 3 = 6 (GS 6) can be a negative predictive factor for Prostate cancer (PCa) indolence. Patients and methods Study was conducted on a retrospective cohort of 123 PCa patients with initial total PSA % 10 ng/ml, number of needle biopsy specimens % 8, GS 6 on biopsy and T1/T2 estimated clinical stage who underwent laparoscopic radical prostatectomy and whose archived formalin-fixed and paraffin-embedded (FFPE) prostate needle biopsy specimens were used for additional immunohistochemistry staining for detection of NF-%B p65. Both cytoplasmic and nuclear NF-%B p65 expression in biopsy cores with PCa were correlated with postoperative pathological stage, positive surgical margins, GS and biochemical progression of disease. Results After follow-up of 66 months, biochemical progression (PSA % 0.2 ng/ml) occurred in 6 (5.1%) patients, 3 (50%) with GS 6 and 3 (50%) with GS 7 after radical prostatectomy. Both cytoplasmic and nuclear NF-%B p65 expressions were not significantly associated with pathological stage, positive surgical margin and postoperative GS. Patients with positive cytoplasmic NF-kB reaction had significantly more frequent biochemical progression than those with negative cytoplasmic NF-kB reaction with PSA 0.2 ng/ml as cutoff point (p = 0.015) and a trend towards more biochemical progression with PSA % 0.05 ng/ml as cutoff point (p = 0.068). Conclusions Cytoplasmic expression of NF-%B is associated with more biochemical progression and might be an independent prognostic factor for recurrence-free survival (RFS), but further studies including larger patient cohorts are needed to confirm these initial results. prostate cancer needle biopsy nuclear factor-kappa beta Gleason rak prostate igelna biopsija nuklearni faktor kappa beta Gleason true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-07-12 10:38:00 2024-07-12 10:38:01 2024-07-13 03:49:23 0000-00-00 00:00:00 2020 0 0 str. 194-200, VII no. 2 Vol. 54 Jun. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-06-01 616.6 1318-2099 10.2478/raon-2020-0019 13549827 https://content.sciendo.com/view/journals/raon/54/2/article-p194.xml 1 0fc76bad-402a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26565 RAZ_Zupancic_Marko_2020.pdf RAZ_Zupancic_Marko_2020.pdf 1 813AAD70A49A965974C09E368D16CC77 ae3541352c5be6cbe9e81d04eb45f368973caf3f3ef1a1b79b6efea4fefea8ad 3b1d701a-402a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26566 Onkološki inštitut Ljubljana 0 0 0