20.500.12556/DiRROS-19271 Studying cell death initiation using a digital microscope Hypersensitive response (HR)-conferred resistance is an effective defense response that can be determined by the N resistance genes. HR is manifested as the formation of cell death zones on inoculated leaves. Here, a protocol for studying the rate of cell death initiation by imaging inoculated leaves in the time between the cell death initiation and the cell death appearance using a digital microscope is presented. The digital microscope enables a continuous imaging process in desired intervals, which allows an accurate determination of cell death initiation rate up to minutes exactly, as opposed to hours in traditional methods. Imaging with the digital microscope is also independent of light and can therefore be used during day and night without disturbing the circadian rhythm of the plant. Different pathosystems resulting in programmed cell death development could be studied using this protocol with minor modifications. Overall, the protocol thus allows simple, accurate, and inexpensive identification of cell death initiation rate. digital microscope cell death inoculated leaves digitalni mikroskop celična smrt inokulirani listi true false true Angleški jezik Slovenski jezik Neznano 2024-07-12 03:38:49 2024-07-12 12:25:58 2024-07-13 03:49:22 0000-00-00 00:00:00 2023 0 0 Nasl. z nasl. zaslona; Opis vira z dne 13. 12. 2023; str. 1-9 [article no.] ǂ3027 Vol. 201 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-11-10 577.2 1940-087X 10.3791/65824 177346819 https://www.jove.com/t/65824/studying-cell-death-initiation-using-a-digital-microscope 1 7d1cfe59-3fef-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26531 RAZ_Arnsek_Tina_2023.pdf RAZ_Arnsek_Tina_2023.pdf 1 13C0FBCA9F2F84C1EE478248732BCCC6 17fb6535b98072e5dfb069a71cb9c526fbdd201b83693b6d3f003e893691c0a0 a2b96a0f-4039-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26593 Nacionalni inštitut za biologijo 0 0 0