20.500.12556/DiRROS-19265 Inter-comparison of marine microbiome sampling protocols Research on marine microbial communities is growing, but studies are hard to compare because of variation in seawater sampling protocols. To help researchers in the inter-comparison of studies that use different seawater sampling methodologies, as well as to help them design future sampling campaigns, we developed the EuroMarine Open Science Exploration initiative (EMOSE). Within the EMOSE framework, we sampled thousands of liters of seawater from a single station in the NW Mediterranean Sea (Service d'Observation du Laboratoire Arago [SOLA], Banyuls-sur-Mer), during one single day. The resulting dataset includes multiple seawater processing approaches, encompassing different material-type kinds of filters (cartridge membrane and flat membrane), three different size fractionations (>0.22 µm, 0.22–3 µm, 3–20 µm and >20 µm), and a number of different seawater volumes ranging from 1 L up to 1000 L. We show that the volume of seawater that is filtered does not have a significant effect on prokaryotic and protist diversity, independently of the sequencing strategy. However, there was a clear difference in alpha and beta diversity between size fractions and between these and “whole water” (with no pre-fractionation). Overall, we recommend care when merging data from datasets that use filters of different pore size, but we consider that the type of filter and volume should not act as confounding variables for the tested sequencing strategies. To the best of our knowledge, this is the first time a publicly available dataset effectively allows for the clarification of the impact of marine microbiome methodological options across a wide range of protocols, including large-scale variations in sampled volume. marine microbiome standardized sampling inter-comparison amplicon sequencing microbial diversity seawater sampling morski mikrobiom standardizirano vzorčenje primerjalna analiza sekvenciranje amplikonov mikrobna diverziteta vzorčenje morske vode true false true Angleški jezik Slovenski jezik Neznano 2024-07-12 03:38:32 2024-07-12 11:32:06 2024-07-13 03:49:22 0000-00-00 00:00:00 2023 0 0 Soavtorji: Maria Paola Tomasino, Roberta Piredda, Grazia Marina Quero, Luís Torgo, Julie Poulain, Pierre E. Galand, Jed A. Fuhrman, Alex Mitchell, Tinkara Tinta, Timotej Turk Dermastia ...; Nasl. z nasl. zaslona; Opis vira z dne 22. 8. 2023; str. [1]-16 art. no. ǂ84 Vol. 3 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-08-19 579 2730-6151 10.1038/s43705-023-00278-w 161933059 https://doi.org/10.1038/s43705-023-00278-w 1 7325a288-3fef-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26525 RAZ_Pascoal_Francisco_2023.pdf RAZ_Pascoal_Francisco_2023.pdf 1 7DE999B24491E8BF7A73EAC3DEA22130 c3fa77886885f95a0c5d87fa8194c48f5908e1a27ec84b852cfb1661b5fe7b59 253f5279-4032-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26578 Nacionalni inštitut za biologijo 0 0 0