20.500.12556/DiRROS-19252 Managing the deluge of newly discovered plant viruses and viroids an optimized scientific and regulatory framework for their characterization and risk analysis The advances in high-throughput sequencing (HTS) technologies and bioinformatic tools have provided new opportunities for virus and viroid discovery and diagnostics. Hence, new sequences of viral origin are being discovered and published at a previously unseen rate. Therefore, a collective effort was undertaken to write and propose a framework for prioritizing the biological characterization steps needed after discovering a new plant virus to evaluate its impact at different levels. Even though the proposed approach was widely used, a revision of these guidelines was prepared to consider virus discovery and characterization trends and integrate novel approaches and tools recently published or under development. This updated framework is more adapted to the current rate of virus discovery and provides an improved prioritization for filling knowledge and data gaps. It consists of four distinct steps adapted to include a multi-stakeholder feedback loop. Key improvements include better prioritization and organization of the various steps, earlier data sharing among researchers and involved stakeholders, public database screening, and exploitation of genomic information to predict biological properties. plant viruses and viroids high throughput sequencing (HTS) biological characterization plant health regulatory agencies Pest Risk Analysis (PRA) virus disease rastlinski virusi in viroidi sekvenciranje zdravje rastlin virusna bolezen true false true Angleški jezik Slovenski jezik Neznano 2024-07-12 03:37:54 2024-07-12 10:58:13 2024-07-13 03:49:21 0000-00-00 00:00:00 2023 0 0 Soavtorji: Maryam Khalili, Ayoub Maachi, Mark Paul S. Rivarez, Johan Rollin, Ferran Salavert, Coline Temple, Miguel A. Aranda, Neil Boonham, Marleen Botermans, Thierry Candresse, Adrian Fox, Yolanda Hernando, Denis Kutnjak, Armelle Marais, Françoise Petter, Maja Ravnikar, Ilhem Selmi, Rachid Tahzima, Charlotte Trontin, Thierry Wetzel and Sebastien Massart; Nasl. z nasl. zaslona; Opis vira z dne 13. 6. 2023; str. 1-13 art. 1181562 Vol. 14 May 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-05-30 632 1664-302X 10.3389/fmicb.2023.1181562 155443459 https://doi.org/10.3389/fmicb.2023.1181562 1 5c380170-3fef-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26510 RAZ_Fontdevila_Pareta_Nuria_2023.pdf RAZ_Fontdevila_Pareta_Nuria_2023.pdf 1 503A4AC9F2760812634A52F3BBEF133A 7024be58f1946ed4ee743de0aeead7f8c5cd5bb0bc28f5c90266351266d56ebc 06e1be69-402d-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26571 Nacionalni inštitut za biologijo 0 0 0