20.500.12556/DiRROS-19237 Tomato brown rugose fruit virus in aqueous environments survival and significance of water-mediated transmission Tomato brown rugose fruit virus in aqueous environments – survival and significance of water-mediated transmission Tomato brown rugose fruit virus (ToBRFV) has recently emerged as a major disease of tomatoes and peppers. ToBRFV is a seed- and contact-transmitted virus. In Slovenia, ToBRFV RNA was detected in samples of wastewater, river, and water used to irrigate plants. Even though the source of detected RNA could not be clearly established, this raised the question of the significance of the detection of ToBRFV in water samples and experimental studies were performed to address this question. The data presented here confirm that the release of virus particles from the roots of infected plants is a source of infectious ToBRFV particles in water and that the virus can remain infective up to four weeks in water stored at room temperature, while its RNA can be detected for much longer. These data also indicate that irrigation with ToBRFV-contaminated water can lead to plant infection. In addition, it has been shown that ToBRFV circulated in drain water in commercial tomato greenhouses from other European countries and that an outbreak of ToBRFV can be detected by regular monitoring of drain water. A simple method for concentrating ToBRFV from water samples and a comparison of the sensitivity of different methods, including the determination of the highest ToBRFV dilution still capable of infecting test plants, were also investigated. The results of our studies fill the knowledge gaps in the epidemiology and diagnosis of ToBRFV, by studying the role of water-mediated transmission, and provide a reliable risk assessment to identify critical points for monitoring and control. tomato brown rugose fruit virus tomato hydroponics water-linked epidemiology survival virus rjave grbančavosti paradižnika paradižnik hidroponija epidemiologija true false true Angleški jezik Slovenski jezik Neznano 2024-07-12 03:37:11 2024-07-12 10:29:10 2024-07-13 03:49:20 0000-00-00 00:00:00 2023 0 0 Soavtorji: Katarina Bačnik, Irena Bajde, Jakob Brodarič, Adrian Fox, Ion Gutiérrez-Aguirre, Miha Kitek, Denis Kutnjak, Yue Lin Loh, Olivera Maksimović Carvalho Ferreira, Maja Ravnikar, Elise Vogel, Christine Vos, Ana Vučurović; Nasl. z nasl. zaslona; Opis vira z dne 6. 6. 2023; str. 1-15 art. 1187920 Vol. 14 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-06-02 632 1664-462X 10.3389/fpls.2023.1187920 154635011 https://doi.org/10.3389/fpls.2023.1187920 1 42e2c99a-3fef-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26494 https://www.frontiersin.org/articles/10.3389/fpls.2023.1187920/full#supplementary-material 1 42e794a8-3fef-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26495 RAZ_Mehle_Natasa_2023.pdf RAZ_Mehle_Natasa_2023.pdf 1 04C5E4CC71B1EB3D6A31CE268642421F 94be5dde3f8dad8021e89e52acd3b31b5d794c35cf2e60d22965663bd8e98551 4c7169a6-4029-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26562 Nacionalni inštitut za biologijo Gozdarski inštitut Slovenije 0 0 0