20.500.12556/DiRROS-19232 Influence of alkylthio and arylthio derivatives of tert-butylquinone on the induction of DNA damage in a human hepatocellular carcinoma cell line (HepG2) The aim of this study was to investigate the effects of tert-butylquinone (TBQ) and its alkylthio and arylthio derivatives on DNA in vitro, using acellular and cellular test systems. Direct interaction with DNA was studied using the plasmid pUC19. Cytotoxic (MTS assay) and genotoxic (comet assay and γH2AX focus assays) effects, and their influence on the cell cycle were studied in the HepG2 cell line. Our results show that TBQ and its derivatives did not directly interact with DNA. The strongest cytotoxic effect on the HepG2 cells was observed for the derivative 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone (IC50 64.68 and 55.64 μM at 24-h and 48-h treatment, respectively). The tested derivatives did not significantly influence the cell cycle distribution in the exposed cellular populations. However, all derivatives showed a genotoxic activity stronger than that of TBQ in the comet assay, with 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone producing the strongest effect. The same derivative also induced DNA double-strand breaks in the γH2AX focus assay. TBQ derivatives HepG2 cell line comet assay γH2AX assay cell cycle analysis cytotoxicity derivati TBQ celična linija HepG2 test komet test gH2AX analiza celičnega cikla citotoksičnost true false false Angleški jezik Slovenski jezik Neznano 2024-07-11 11:31:12 2024-07-11 11:31:13 2024-08-08 04:07:49 0000-00-00 00:00:00 2024 0 0 Soavtorji: Stoimir Kolarević, Jovana Jovanović Marić, Margareta Kračun-Kolarević, Bojana Žegura, Alja Štern, Dušan Sladić, Irena Novaković, Branka Vuković-Gačić; Nasl. z nasl. zaslona; Opis vira z dne 2. 7. 2024; str. 1-8 [article no.] ǂ105882 Vol. 99 2024 0000-00-00 PreprintDelovna Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-08-01 577.2 1879-3177 10.1016/j.tiv.2024.105882 200413443 https://www.sciencedirect.com/science/article/pii/S0887233324001127?dgcid=coauthor 1 52c0eb8e-3f68-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26487 RAZ_Djordjevic_Jelena_2024.docx RAZ_Djordjevic_Jelena_2024.docx 1 9386A1E6839152BCD3A6C332D04DFE45 81678e591ab37af9f7b03c5b38667d5e1184194f7368922412deb2e5331c0e13 7870ffaf-549f-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28233 Nacionalni inštitut za biologijo 0 0 0