20.500.12556/DiRROS-19169 Transcription factors gene expression in chronic rhinosinusitis with and without nasal polyps Background. Chronic rhinosinusitis (CRS) current therapeutic approaches still fail in some patients with severe persistent symptoms and recurrences after surgery. We aimed to evaluate the master transcription factors gene expression levels of T cell subtypes in chronic rhinosinusitis with nasal polyps (CRSwNP) and chronic rhinosinusitis without nasal polyps (CRSsNP) that could represent new, up-stream targets for topical DNAzyme treatment. Patients and methods. Twenty-two newly diagnosed CRS patients (14 CRSwNP and 8 CRSsNP) were prospectively biopsied and examined histopathologically. Gene expression levels of T-box transcription factor (T-bet, TBX21), GATA binding protein 3 (GATA3), Retinoic acid-related orphan receptor C (RORC) and Forkhead box P3 (FOXP3) were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). Results. Eosinophilic CRSwNP was characterized by higher level of GATA3 gene expression compared to noneosinophilic CRSwNP, whereas there was no difference in T-bet, RORC and FOXP3 between eosinophilic and noneosinophilic CRSwNP. In CRSsNP, we found simultaneous upregulation of T-bet, GATA3 and RORC gene expression levels in comparison to CRSwNP; meanwhile, there was no difference in FOXP3 gene expression between CRSwNP and CRSsNP. Conclusions. In eosinophilic CRSwNP, we confirmed the type 2 inflammation by elevated GATA3 gene expression level. In CRSsNP, we unexpectedly found simultaneous upregulation of T-bet and GATA3 that is currently unexplained; however, it might originate from activated CD8+ cells, abundant in nasal mucosa of CRSsNP patients. The elevated RORC in CRSsNP could be part of homeostatic nasal immune response that might be better preserved in CRSsNP patients compared to CRSwNP patients. Further data on transcription factors expression rates in CRS phenotypes are needed. chronic rhinosinusitis kronični rinosinuzitis true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-07-05 11:18:45 2024-07-05 11:18:45 2024-07-06 03:42:51 0000-00-00 00:00:00 2019 0 0 Soavtorji: Matija Rijavec, Mira Silar, Ana Koren, Izidor Kern, Irena Hocevar-Boltezar, Peter Korosec; Nasl. z nasl. zaslona; Opis vira z dne 26. 7. 2019; str. 323-330 iss. 3 Vol. 53 Sep. 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2019-09-01 616.21 1581-3207 10.2478/raon-2019-0029 2048522353 https://www.degruyter.com/downloadpdf/j/raon.ahead-of-print/raon-2019-0029/raon-2019-0029.pdf 1 95e47869-3aaf-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26392 RAZ_Soklic_Tanja_2019.pdf RAZ_Soklic_Tanja_2019.pdf 1 D8B94F68E4F1A5A99B70D02640EBFD1F 651803d695b55042daebe9c5e8aa01febb110ae802d8502b59a0afa19a055752 d657acc5-3aaf-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26393 Onkološki inštitut Ljubljana 0 0 0