20.500.12556/DiRROS-19129 The activation of iron deficiency responses of grapevine rootstocks is dependent to the availability of the nitrogen forms Background In viticulture, iron (Fe) chlorosis is a common abiotic stress that impairs plant development and leads to yield and quality losses. Under low availability of the metal, the applied N form (nitrate and ammonium) can play a role in promoting or mitigating Fe deficiency stresses. However, the processes involved are not clear in grapevine. Therefore, the aim of this study was to investigate the response of two grapevine rootstocks to the interaction between N forms and Fe uptake. This process was evaluated in a hydroponic experiment using two ungrafted grapevine rootstocks Fercal (Vitis berlandieri x V. vinifera) tolerant to deficiency induced Fe chlorosis and Couderc 3309 (V. riparia x V. rupestris) susceptible to deficiency induced Fe chlorosis. Results The results could differentiate Fe deficiency effects, N-forms effects, and rootstock effects. Interveinal chlorosis of young leaves appeared earlier on 3309 C from the second week of treatment with NO3 −/NH4 + (1:0)/- Fe, while Fercal leaves showed less severe symptoms after four weeks of treatment, corresponding to decreased chlorophyll concentrations lowered by 75% in 3309 C and 57% in Fercal. Ferric chelate reductase (FCR) activity was by trend enhanced under Fe deficiency in Fercal with both N combinations, whereas 3309 C showed an increase in FCR activity under Fe deficiency only with NO3 −/NH4 + (1:1) treatment. With the transcriptome analysis, Gene Ontology (GO) revealed multiple biological processes and molecular functions that were significantly regulated in grapevine rootstocks under Fe-deficient conditions, with more genes regulated in Fercal responses, especially when both forms of N were supplied. Furthermore, the expression of genes involved in the auxin and abscisic acid metabolic pathways was markedly increased by the equal supply of both forms of N under Fe deficiency conditions. In addition, changes in the expression of genes related to Fe uptake, regulation, and transport reflected the different responses of the two grapevine rootstocks to different N forms. Conclusions Results show a clear contribution of N forms to the response of the two grapevine rootstocks under Fe deficiency, highlighting the importance of providing both N forms (nitrate and ammonium) in an appropriate ratio in order to ease the rootstock responses to Fe deficiency. vitis iron uptake chlorosis nitrate ammonium RNA-Seq železo vinska trta amonij RNA nitrat true false true Angleški jezik Slovenski jezik Neznano 2024-06-19 15:52:02 2024-06-19 15:52:02 2024-06-20 03:37:43 0000-00-00 00:00:00 2024 0 0 Soavtorji: Rebeka Strah, Arianna Lodovici, Petr Vojta, Federica De Berardinis, Jörg Ziegler, Maruša Pompe Novak, Laura Zanin, Nicola Tomasi, Astrid Forneck & Michaela Griesser; Nasl. z nasl. zaslona; Opis vira z dne 5. 4. 2024; str. 1-23 [article no.] 218 Vol. 24 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2024-03-26 632 1471-2229 10.1186/s12870-024-04906-y 191557635 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-024-04906-y 1 1d0c1fec-2e43-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26321 RAZ_Khalil_Sarhan_2024.pdf RAZ_Khalil_Sarhan_2024.pdf 1 46548A8618BF1A29ADC89B84FE041400 47015c23854a6b93273d568c225dd754d49bdcfd1091d0952f9ebf4b01fa82bc 909b092a-2e43-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26322 Nacionalni inštitut za biologijo 0 0 0