20.500.12556/DiRROS-19053 Biocompatible polyelectrolyte multilayers with copper oxide and zinc oxide nanoparticles for inhibiting bacterial growth The prevalence of bacterial infections presents a significant challenge in the medical field, demanding effective strategies to impede bacterial adhesion and growth on various surfaces. The conducted study investigates the efficacy of polyelectrolyte multilayers─comprising poly(allylamine hydrochloride) (PAH) and alginate (ALG)─embedded with zinc oxide (ZnO) and copper oxide (CuO) nanoparticles (NPs) to inhibit bacterial adhesion on stainless-steel surfaces. Surface characterization involved zeta potential, contact angle, and roughness assessments. The effect of NP composition, size, and morphology in conjunction with polycation or polyanion terminating multilayers was evaluated against planktonic and surface-adhered Escherichia coli (E. coli) cells. Surfaces with the positively charged PAH-terminating multilayer displayed higher water contact angles (≈ 63°) than the negatively charged ALG-terminating multilayers (≈ 45°). Multilayers containing ZnO NPs showed a significant inhibition of planktonic E. coli growth, >99%. Moreover, complete growth inhibition of surface-adhered E. coli was achieved for multilayers containing both ZnO and CuO. Due to their larger specific surface area, rod-like ZnO NPs displayed higher antibacterial activity. The samples with ALG as the terminating layer showed more substantial antibacterial properties than samples with PAH as the terminating layer. Biocompatibility tests on immortalized human keratinocyte cells revealed good compatibility with multilayers incorporating NPs. In summary, this study underscores the potential of ZnO and CuO NPs within PAH/ALG multilayers for antibacterial applications without compromising their cytocompatibility. polyelectrolyte multilayers alginate poly(allylamine hydrochloride) CuO ZnO nanoparticles Escherichia coli večplastni polielektroliti alginat poli(alilamin hidroklorid) CuO ZnO nanodelci Escherichia coli true false true American Chemical Society Angleški jezik Slovenski jezik © 2024 The Authors. Neznano 2024-06-06 11:11:43 2024-06-06 11:19:42 2024-08-14 08:43:02 0000-00-00 00:00:00 2024 0 0 str. 12207-13820 issue 11 Vol. 7 June 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-05-16 577:579 2574-0970 10.1021/acsanm.4c00981 195799811 https://pubs.acs.org/doi/10.1021/acsanm.4c00981 1 cba86ed8-23e4-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26188 RAZ_Matijakovic_Mlinaric_Nives_2024.pdf RAZ_Matijakovic_Mlinaric_Nives_2024.pdf 1 4EEA953C3C6D66A4B3681F14D877864A 695c942ce6f17c0ca8a37606e3756e0bedcfa312b84ce8187299d9afc5c9615b 5711b6b9-23e5-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26189 RAZ_Matijakovic_Mlinaric_Nives_2024.pdf RAZ_Matijakovic_Mlinaric_Nives_2024.pdf 1 AB525F563B3F21350ADFB18E6015F7FB 044bc7287b195f88e6cc1408134324efd2e1c61cd31c0879b3e6a3e62e2add88 9641d0de-23e5-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26190 Zavod za gradbeništvo Slovenije 0 0 0