20.500.12556/DiRROS-19037 Distribution of toxigenic cyanobacteria in Alpine lakes and rivers as revealed by molecular screening The increasing frequency of cyanobacteria blooms in waterbodies caused by ecosystem eutrophication could endanger human health. This risk can be mitigated by effective monitoring incorporating molecular methods. To date, most molecular studies on toxigenic cyanobacteria have been limited to microcystins (MCs), disregarding other cyanotoxins, to freshwater planktic habitats while ignoring benthic habitats, and to limited geographic areas (usually one or a few specific waterbodies). In this study, we used PCR-based methods including PCR product sequencing and chemical-analytical methods (LC-MS/MS) to screen many plankton (n = 123) and biofilm samples (n = 113) originating from 29 Alpine lakes and 18 rivers for their cyanotoxin production potential. Both mcyE (indicating MC synthesis) and anaC (indicating anatoxin (ATX) synthesis) gene fragments were able to qualitatively predict MC or ATX occurrence. The abundance of mcyE gene fragments was significantly related to MC concentrations in plankton samples (R2 = 0.61). mcyE gene fragments indicative of MC synthesis were most abundant in planktic samples (65 %) and were assigned to the genera Planktothrix and Microcystis. However, mcyE rarely occurred in biofilms of lakes and rivers, i.e., 4 % and 5 %, respectively, and were assigned to Microcystis, Planktothrix, and Nostoc. In contrast, anaC gene fragments occurred frequently in planktic samples (14 % assigned to Tychonema, Phormidium (Microcoleus), and Oscillatoria), but also in biofilms of lakes (49 %) and rivers (18 %) and were assigned to the genera Phormidium, Oscillatoria, and Nostocales. The cyrJ gene fragment indicating cylindrospermopsin synthesis occurred only once in plankton (assigned to Dolichospermum), while saxitoxin synthesis potential was not detected. For plankton samples, monomictic and less eutrophic conditions were positively related to mcyE/MC occurrence frequency, while oligomictic conditions were related to anaC/ATX frequency. The anaC/ATX frequency in biofilm was related to the lake habitats generally showing higher biodiversity as revealed from metabarcoding in a parallel study. cyanotoxins planktic and benthic habitats water quality monitoring early warning toxigenic cyanobacteria European Alps cianotoksini planktonski in bentoški habitati monitoring kakovosti voda zgodnje zaznavanje toksigene cianobakterije Alpe true false true Angleški jezik Slovenski jezik Neznano 2024-06-03 12:46:12 2024-06-03 12:46:13 2024-06-04 03:38:30 0000-00-00 00:00:00 2024 0 0 Soavtorji: Leonardo Cerasino, Adriano Boscaini, Camilla Capelli, Claudia Greco, Aleksandra Krivograd Klemenčič, Ute Mischke, Nico Salmaso, Rainer Kurmayer; str. [1]-11 [art. no.] ǂ121783 Vol. 258 Jul. 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2024-05-15 579 0043-1354 10.1016/j.watres.2024.121783 197565187 https://doi.org/10.1016/j.watres.2024.121783 1 8134ee85-2196-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26131 RAZ_Jablonska_Masa_2024.pdf RAZ_Jablonska_Masa_2024.pdf 1 BA49893ED6F80405D8E67856E9057E9C 7dd8fce54fe39c57962918cbdbe956358f872b3bc9607a366ad8c02798ad0813 9f8a3fd1-2196-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26132 Nacionalni inštitut za biologijo 0 0 0