20.500.12556/DiRROS-18982 Genotoxicity and heating performance of VxFe3-xO4 nanoparticles in health applications The applications of magnetic nanoparticles (MNPs) as biocatalysts in different biomedical areas have been evolved very recently. One of the main challenges in this field is to design affective MNPs surfaces with catalytically active atomic centres, while producing minimal toxicological side effects on the hosting cell or tissues. MNPs of vanadium spinel ferrite (VFe2O4) are a promising material for mimicking the action of natural enzymes in degrading harmful substrates due to the presence of active V5+ centres. However, the toxicity of this material has not been yet studied in detail enough to grant biomedical safety. In this work, we have extensively measured the structural, compositional, and magnetic properties of a series of VxFe3-xO4 spinel ferrite MNPs to assess the surface composition and oxidation state of V atoms, and also performed systematic and extensive in vitro cytotoxicity and genotoxicity testing required to assess their safety in potential clinical applications. We could establish the presence of V5+ at the particle surface even in water-based colloidal samples at pH 7, as well as different amounts of V2+ and V3+ substitution at the A and B sites of the spinel structure. All samples showed large heating efficiency with Specific Loss Power values up to 400 W/g (H0 = 30 kA/m; f = 700 kHz). Samples analysed for safety in human hepatocellular carcinoma (HepG2) cell line with up to 24h of exposure showed that these MNPs did not induce major genomic abnormalities such as micronuclei, nuclear buds, or nucleoplasmic bridges (MNIs, NBUDs, and NPBs), nor did they cause DNA double-strand breaks (DSBs) or aneugenic effects—types of damage considered most harmful to cellular genetic material. The present study is an essential step towards the use of these type of nanomaterials in any biomedical or clinical application. magnetic nanoparticles vanadium ferrite cytotoxicity genotoxicity specific power absorption cell viability magnetni nanodelci vanadijev ferit citotoksičnost genotoksičnost specifična absorpcijska moč celična živost true false true Angleški jezik Slovenski jezik Neznano 2024-05-23 12:22:02 2024-05-23 12:22:02 2024-05-24 03:40:31 0000-00-00 00:00:00 2024 0 0 Soavtorji: Amaia Sáenz- Hernández, A. C. Moreno Maldonado, Jesús A. Fuentes-García, Jorge M. Nuñez, Bojana Žegura, Alja Štern, Katja Kološa, Iza Rozman, Teobaldo E. Torres, Gerardo F. Goya; str. [1]-13 [art. no.] ǂ110977 Vol. 394 1 May 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2024-03-24 577 0009-2797 10.1016/j.cbi.2024.110977 190594819 https://doi.org/10.1016/j.cbi.2024.110977 1 4d7ee38c-18ee-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26025 RAZ_Sanz-Sague_Beatriz_2024.pdf RAZ_Sanz-Sague_Beatriz_2024.pdf 1 4D926F6E26809354A5A80AE33B6BEE21 06bfde4cc9c3b158fb631741e55e20245921c47c5a868198d10a4f471864d6c6 6fd0ab7d-18ee-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26026 Nacionalni inštitut za biologijo 0 0 0