20.500.12556/DiRROS-18941 Bacterial degradation of ctenophore Mnemiopsis leidyi organic matter Blooms of gelatinous zooplankton, an important source of protein-rich biomass in coastal waters, often collapse rapidly, releasing large amounts of labile detrital organic matter (OM) into the surrounding water. Although these blooms have the potential to cause major perturbations in the marine ecosystem, their effects on the microbial community and hence on the biogeochemical cycles have yet to be elucidated. We conducted microcosm experiments simulating the scenario experienced by coastal bacterial communities after the decay of a ctenophore (Mnemiopsis leidyi) bloom in the northern Adriatic Sea. Within 24 h, a rapid response of bacterial communities to the M. leidyi OM was observed, characterized by elevated bacterial biomass production and respiration rates. However, compared to our previous microcosm study of jellyfish (Aurelia aurita s.l.), M. leidyi OM degradation was characterized by significantly lower bacterial growth efficiency, meaning that the carbon stored in the OM was mostly respired. Combined metagenomic and metaproteomic analysis indicated that the degradation activity was mainly performed by Pseudoalteromonas, producing a large amount of proteolytic extracellular enzymes and exhibiting high metabolic activity. Interestingly, the reconstructed metagenome-assembled genome (MAG) of Pseudoalteromonas phenolica was almost identical (average nucleotide identity >99%) to the MAG previously reconstructed in our A. aurita microcosm study, despite the fundamental genetic and biochemical differences of the two gelatinous zooplankton species. Taken together, our data suggest that blooms of different gelatinous zooplankton are likely triggering a consistent response from natural bacterial communities, with specific bacterial lineages driving the remineralization of the gelatinous OM. jellyfish proteases bacterioplankton ocean biogeochemistry meduze protease bacterioplankton biogeokemija morja true false true Angleški jezik Slovenski jezik Neznano 2024-05-16 14:35:01 2024-05-16 14:35:01 2024-05-20 08:34:19 0000-00-00 00:00:00 2024 0 0 Soavtorji: Jennifer H. Hennenfeind, Chie Amano, Zihao Zhao, Katja Klun, Gerhard J. Herndl, Tinkara Tinta; Nasl. z nasl. zaslona; Opis vira z dne 13. 2. 2024; str. 1-22 iss. 2 Vol. 9 Feb. 2024 0000-00-00 Zaloznikova NiDoloceno apc 0000-00-00 0000-00-00 2024-01-23 577 2379-5077 10.1128/msystems.01264-23 184957699 https://doi.org/10.1128/msystems.01264-23 1 b88e1fd4-1380-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25963 RAZ_Fadeev_Eduard_2024.pdf RAZ_Fadeev_Eduard_2024.pdf 1 C7B7DFD10F1A1A0BFE5223C40E805BBB f25932a9801af2bcefce347f5efad07ad7d84f00a0727d87f3dd87d97c77aeea 117ecf0a-1381-11ef-9c9e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25964 Nacionalni inštitut za biologijo 0 0 0