20.500.12556/DiRROS-18660 Clonality analysis of lymphoid proliferations using the BIOMED-2 clonality assays a single institution experience Background. Clonality determination in patients with lymphoproliferative disorders can improve the final diagnosis.The aim of our study was to evaluate the applicative value of standardized BIOMED-2 gene clonality assay protocolsfor the analysis of clonality of lymphocytes in a group of different lymphoid proliferations.Materials and methods. With this purpose, 121 specimens from 91 patients with suspected lymphoproliferationssubmitted for routine diagnostics from January to December 2011 were retrospectively analyzed. According to thefinal diagnosis, our series comprised 32 cases of B-cell lymphomas, 38 cases of non-Hodgkins T-cell lymphomas and51 cases of reactive lymphoid proliferations. Clonality testing was performed using the BIOMED-2 clonality assays.Results. The determined sensitivity of the TCR assay was 91.9%, while the sensitivity of the IGH assay was 74.2%. Thedetermined specificity of the IGH assay was 73.3% in the group of lymphomas and 87.2% in the group of reactivelesions. The determined specificity of the TCR assay was 62.5% in the group of lymphomas and 54.3% in the group ofreactive lesions.Conclusions. In the present study, we confirmed the utility of standardized BIOMED-2 clonality assays for the detectionof clonality in a routine diagnostical setting of non-Hodgkins lymphomas. Reactions for the detection of thecomplete IGH rearrangements and reactions for the detection of the TCR rearrangements are a good choice forclonality testing of a wide range of lymphoid proliferations and specimen types while the reactions for the detectionof incomplete IGH rearrangements have not shown any additional diagnostic value. Biomed-2 clonality analysis lymphomas IGH rearrangement TCR rearrangement klonalna anliza limfomi standardizirane metode IGH preureditev TCR preureditev true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-04-11 10:58:22 2024-04-11 10:58:23 2024-04-12 03:37:21 0000-00-00 00:00:00 2014 0 0 str. 155-162 no. 2 Vol. 48 Jun. 2014 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2014-06-01 602.6/.7:616.1 1318-2099 10.2478/raon-2013-0072 1715835 RAZ_Kokovic_Ira_2014.pdf RAZ_Kokovic_Ira_2014.pdf 1 6D86DC06D5590232C4277EA61F6C7798 4c09c2d87df4370a9e8b216dabc2cfec0443263d67cf61e6924bf7c544b6e5b3 16a7a9a1-f7d1-11ee-ae20-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25491 Onkološki inštitut Ljubljana 0 0 0