20.500.12556/DiRROS-18591 Heterogeneity of uroplakin localization in human normal urothelium, papilloma and papillary carcinoma Background. Uroplakins are differentiation-related membrane proteins of urothelium. We compared uroplakin expression and ultrastructural localization in human normal urothelium, papilloma and papillary carcinoma. Because of high recurrence rate of these tumours, treated by transurethral resection, we investigated urothelial tumour, resection border and uninvolved urothelium.Patients and methods. Urinary bladder samples were obtained from tumour free control subjects and patients with papilloma and papillary carcinoma. Immunohistochemical and immunoelectron labelling of uroplakins were performed.Results. In normal human urothelium with continuous uroplakin-positive superficial cell layer uroplakins were localized to flattened mature fusiform vesicles and apical plasma membrane of umbrella cells. Diverse uroplakin expression was found in papilloma and papillary carcinoma. Three aberrant differentiation stages of urothelial cells, not found in normal urothelium, were recognized in tumours. Diverse uroplakin expression and aberrant differentiation were occasionally found in resection border and in uninvolved urothelium.Conclusions. We demonstrated here that uroplakin expression and localization in urothelial tumours is altered when compared to normal urothelium. In patients with papilloma and papillary carcinoma immunolabelling of uroplakins at ultrastructural level shows aberrant urothelial differentiation. It is possible that aberrant differentiation stages of urothelial cells in resection border and in uninvolved urothelium contribute to high recurrence rate. human urothelium papilloma papillary carcinoma človeški urotelij papiloma papilarni karcinom true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-04-03 14:23:53 2024-04-03 14:23:54 2024-04-04 03:35:24 0000-00-00 00:00:00 2013 0 0 Izvleček v slov. na str. II; str. 338-345, II no. 4 a1404, ale1404 dec. 2013 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2013-12-01 616-006 1318-2099 10.2478/raon-2013-0052 30908121 30907609 http://www.degruyter.com/view/j/raon.2013.47.issue-4/raon-2013-0052/raon-2013-0052.xml?format=INT 1 abe8b9b1-f1ac-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25364 RAZ_Zupancic_Dasa_2013.pdf RAZ_Zupancic_Dasa_2013.pdf 1 ECCE496BF5252159A052351D2986084C b3c42a0389a1afabf696d4523746002cd54e04ca9fc6f0f2217f0b7a961567ac e2c3c20e-f1ad-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25366 Onkološki inštitut Ljubljana 0 0 0