20.500.12556/DiRROS-18590 Expansive growth of two glioblastoma stem-like cell lines is mediated by bFGF and not by EGF Background. Patient-derived glioblastoma (GBM) stem-like cells (GSCs) represent a valuable model for basic and therapeutic research. GSCs are usually propagated in serum-free Neural Basal medium supplemented with bFGF and EGF. Yet, the exact influence of these growth factors on GSCs is still unclear. Recently it was suggested that GBM stemlike cells with amplified EGFR should be cultured in stem cell medium without EGF, as the presence of EGF induced rapid loss of EGFR amplification. However, patient biopsies are usually taken into culture before their genomic profiles are defined. Thus, an important question remains whether GBM cells without EGFR amplification also can be cultured in stem cell medium without EGF.Meterials and methods. To address this question, we used two heterogeneous glioblastoma GSC lines (NCH421k and NCH644) that lack EGFR amplification.Results. Although both cell lines showed very low EGFR expression under standard growth conditions, bFGF stimulation induced higher expression of EGFR in NCH644. In both cell lines, expression of the stem cell markers nestin and CD133 was higher upon stimulation with bFGF compared to EGF. Importantly, bFGF stimulated the growth of both cell lines, whereas EGF had no effect. We verified that the growth stimulation by bFGF was either mediated by proliferation (NCH421k) or resistance to apoptosis (NCH644).Conclusions. We demonstrate that GSC cultures without EGFR amplification can be maintained and expanded with bFGF, while the addition of EGF has no significant effect and therefore can be omitted. glioblastoma stem cell cultures bFGF glioblastoma kulture matičnih celic bFGF true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-04-03 14:21:55 2024-04-03 14:21:56 2024-04-04 03:35:24 0000-00-00 00:00:00 2013 0 0 Izvleček v slov. na str. II; str. 330-337, II no. 4 Vol. 47 dec. 2013 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2013-12-01 616.1 1318-2099 10.2478/raon-2013-0063 30906329 30907609 http://www.degruyter.com/view/j/raon.2013.47.issue-4/raon-2013-0063/raon-2013-0063.xml 1 661183b0-f1ac-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25362 RAZ_Podergajs_Neza_2013.pdf RAZ_Podergajs_Neza_2013.pdf 1 7A4126C052F0159D875CA3F3AE0AB26A 565c22103e1a50c5556a5253724b39299b94820b1859ec69125ca833be5c2993 8fc383dd-f1ac-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25363 Onkološki inštitut Ljubljana 0 0 0