20.500.12556/DiRROS-18584 Transcriptome-informed identification and characterization of Planococcus citri cis- and trans-isoprenyl diphosphate synthase genes Insect physiology and reproduction depend on several terpenoid compounds, whose biosynthesis is mainly unknown. One enigmatic group of insect monoterpenoids are mealybug sex pheromones, presumably resulting from the irregular coupling activity of unidentified isoprenyl diphosphate synthases (IDSs). Here, we performed a comprehensive search for IDS coding sequences of the pest mealybug Planococcus citri. We queried the available genomic and newly generated short- and long-read P. citri transcriptomic data and identified 18 putative IDS genes, whose phylogenetic analysis indicates several gene family expansion events. In vitro testing confirmed regular short-chain coupling activity with five gene products. With the candidate with highest IDS activity, we also detected low amounts of irregular coupling products, and determined amino acid residues important for chain-length preference and irregular coupling activity. This work therefore provides an important foundation for deciphering terpenoid biosynthesis in mealybugs, including the sex pheromone biosynthesis in P. citri. insect pheromones isoprenyl diphosphate synthase monoterpenes phylogenetic analysis žuželčji feromoni izoprenil difosfat sintaze monoterpeni filogenetska analiza Planococcus citri true false true Angleški jezik Latinski jezik Neznano 2024-03-29 11:59:08 2024-03-29 11:59:08 2024-05-23 11:45:06 0000-00-00 00:00:00 2024 0 0 Soavtorji: Gerasymenko, M. Iryna; Petek, Marko; Haumman, Elisabeth; Vacas, Sandra; Kallam, Kalyani; Gianoglio, Silvia; Navarro-Llopis, Vicente; Heethoff, Michael; Navarro Fuertes, Ismael; Patron, Nicola; Orzáez, Diego; Gruden, Kristina; Warzecha, Heribert; Baebler, Špela; Nasl. z nasl. zaslona; Opis vira z dne 21. 3. 2024; str. 1-18 [art. no.] ǂ109441 iss. 4 Apr. 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2024-03-06 577.2 2589-0042 10.1016/j.isci.2024.109441 189713411 https://doi.org/10.1016/j.isci.2024.109441 1 fda5afff-edb2-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25344 RAZ_Jutersek_Mojca_2024.pdf RAZ_Jutersek_Mojca_2024.pdf 1 271EE9E3D169614CAC6B53DD0C0515B2 36891127d995d430963cf716415115fb663e8880eaccf821ff4279188fe073ed 53c93c81-edb4-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25345 Nacionalni inštitut za biologijo 0 0 0