20.500.12556/DiRROS-18581 Keep it simple improving the ex situ culture of Cystoseira s.l. to restore macroalgal forests Keep it simple: improving the ex situ culture of Cystoseira s.l. to restore macroalgal forests Brown algae from genus Cystoseira s.l. form dense underwater forests that represent the most productive areas in the Mediterranean Sea. Due to the combined effects of global and local stressors such as climate change, urbanization, and herbivore outbreaks, there has been a severe decline in brown algal forests in the Mediterranean Sea. Natural recovery of depleted sites is unlikely due to the low dispersal capacity of these species, and efficient techniques to restore such habitats are needed. In this context, the aims of our study were (1) to improve and simplify the current ex situ laboratory protocol for the cultivation of Gongolaria barbata by testing the feasibility of some cost-effective and time-efficient techniques on two donor sites of G. barbata and (2) to evaluate the survival and growth of young thalli during the laboratory phase and during the most critical five months after out-planting. Specifically, the following ex situ cultivation methods were tested: (A) cultivation on clay tiles in mesocosms with culture water prepared by three different procedures (a) filtered seawater with a 0.22 μm filter membrane, (b) filtered seawater with a 0.7 μm filter membrane (GF), and (c) UV-sterilized water, and (B) cultivation on clay tiles in open laboratory systems. After two weeks, all thalli were fixed to plastic lantern net baskets suspended at a depth of 2 m in the coastal sea (hybrid method), and the algal success was monitored in relation to the different donor sites and cultivation protocol. The satisfactory results of this study indicate that UV-sterilized water is suitable for the cultivation of G. barbata in mesocosm, which significantly reduces the cost of the laboratory phase. This opens the possibility of numerous and frequent algal cultures during the reproductive period of the species. Additionally, if the young thalli remain in the lantern net baskets for an extended period of several months, they can grow significantly in the marine environment without being exposed to pressure from herbivorous fish. gojenje ex situ obnova habitata izboljšanje metodologije gozdički rjavih alg ex situ cultivation mesocosm open system method improvement brown algal forests restoration Gongolaria barbata true false true Angleški jezik Latinski jezik Neznano 2024-03-29 09:25:49 2024-03-29 09:25:51 2024-05-23 11:46:00 0000-00-00 00:00:00 2023 0 0 Soavtorji: Valentina Pitacco, Domen Trkov, Leon Lojze Zamuda, Annalisa Falace, and Martina Orlando-Bonaca; Nasl. z nasl. zaslona; Opis vira z dne 14. 7. 2023; str. [1]-18 art. 2615 iss. 14 Jul. 2023 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2023-07-11 574 2223-7747 10.3390/plants12142615 158892803 https://doi.org/10.3390/plants12142615 1 92f84b54-ed9d-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25338 Lokovsek-2023-Keep-it-simple-improving-the-ex-sit.pdf Lokovsek-2023-Keep-it-simple-improving-the-ex-sit.pdf 1 1BC5BDEDF7D3636CBB6B84A489F94D48 ee2fc43811c416ff3be807d0e80ee68219045f65daff04808e70c85d99c4a819 de137d50-ed9d-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25339 Nacionalni inštitut za biologijo 0 0 0