20.500.12556/DiRROS-18514 Assessment of the tumourigenic and metastatic properties of SK-MEL28 melanoma cells surviving electrochemotherapy with bleomycin Določitev tumorigenih in metastatskih lastnosti melanomskih celic SK-MEL28 po preživetju elektrokemoterapije z bleomicinom Background. Electrochemotherapy is a local treatment combining chemotherapy and electroporation and is highly effective treatment approach for subcutaneous tumours of various histologies. Contrary to surgery and radiation, the effect of electrochemotherapy on metastatic potential of tumour cells has not been extensively studied. The aim of the study was to evaluate the effect of electrochemotherapy with bleomycin on the metastatic potential of human melanoma cells in vitro. Materials and methods. Viable cells 48 hours after electrochemotherapy were tested for their ability to migrate and invade through Matrigel coated porous membrane. In addition, microarray analysis and quantitative Real-Time PCR were used to detect changes in gene expression after electrochemotherapy. Results. Cell migration and invasion were not changed in melanoma cells surviving electrochemotherapy.Interestingly, only a low number of tumourigenesis related genes was differentially expressed after electrochemotherapy. Conclusions. Our data suggest that metastatic potential of human melanoma cells is not affected by electrochemotherapy with bleomycin, confirming safe role of electrochemotherapy in the clinics. elektrokemoterapija tumorne celice bleomicin melanom true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-03-21 12:18:27 2024-03-21 12:18:28 2024-03-22 03:48:33 0000-00-00 00:00:00 2012 0 0 Soavtorji: Gregor Serša, Vid Mlakar, Damjan Glavač, Maja Čemažar; str. 32-45, III no. 1 Vol. 46 2012 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2012-01-01 616-006-08 1318-2099 10.2478/v10019-012-0010-6 3203441 http://versita.metapress.com/content/24337t420311w012/fulltext.pdf 1 c23bcbd8-e774-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25229 RAZ_Todorovic_Vesna_2012.pdf RAZ_Todorovic_Vesna_2012.pdf 1 6CBEB88B786C58B8A9BB1BBCF8D8120A 730dfb65c4c54b44ed66971c62873d842a5346eea19440cbd733fd4371a8e18f ef3e9c84-e774-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25230 Onkološki inštitut Ljubljana 0 0 0