20.500.12556/DiRROS-18508 Gene immunotherapy of colon carcinoma with IL-2 and IL-12 using gene electrotransfer Gene immunotherapy has become an important approach in the treatment of cancer. One example is the introduction of genes encoding immunostimulatory cytokines, such as interleukin 2 and interleukin 12, which stimulate immune cells in tumours. The aim of our study was to determine the effects of gene electrotransfer of plasmids encoding interleukin 2 and interleukin 12 individually and in combination in the CT26 murine colon carcinoma cell line in mice. In the in vitro experiment, the pulse protocol that resulted in the highest expression of IL-2 and IL-12 mRNA and proteins was used for the in vivo part. In vivo, tumour growth delay and also complete response were observed in the group treated with the plasmid combination. Compared to the control group, the highest levels of various immunostimulatory cytokines and increased immune infiltration were observed in the combination group. Long-term anti-tumour immunity was observed in the combination group after tumour re-challenge. In conclusion, our combination therapy efficiently eradicated CT26 colon carcinoma in mice and also generated strong anti-tumour immune memory. colon carcinoma gene electrotransfer gene immunotherapy rak debelega črevesa elektroprenos genov genska imunoterapija true false true MDPI Basel, Switzerland Angleški jezik Slovenski jezik by Authors Neznano 2024-03-21 09:03:18 2024-03-21 09:03:18 2024-03-22 03:48:33 0000-00-00 00:00:00 2023 0 0 Nasl. z nasl. zaslona; Opis vira z dne 25. 8. 2023; str. 12900-1-12900-25 no. 16 Vol. 24 2023 0000-00-00 Zaloznikova Objavljeno apc 2023-07-20 2023-08-15 2023-08-17 602.6/.7 1422-0067 10.3390/ijms241612900 162334211 https://www.mdpi.com/1422-0067/24/16/12900 1 7b8187d3-e759-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25218 RAZ_Komel_Tilen_2023.pdf RAZ_Komel_Tilen_2023.pdf 1 8C2E5CE838ECAE708FAF67628D645AB9 32022c2c9e5f87f58a3e88affd2d63a390484fd1868dcf4ba4edde7cd27f71f1 ff3df0aa-e75a-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25219 Onkološki inštitut Ljubljana 0 0 0