20.500.12556/DiRROS-18457 Cathepsin H indirectly regulates morphogenetic protein-4 (BMP-4) in various human cell lines Katepsin H posredno regulira kostni morfogenetski protein 4 (BMP-4) v človeških celičnih linijah Background. Cathepsin H is a cysteine protease considered to play a major role in tumor progression, however, its precise function in tumorigenesis is unclear. Cathepsin H was recently proposed to be involved in processing of bone morphogenetic protein 4 (BMP-4) in mice. In order to clarify whether cathepsin H also regulates BMP-4 in humans, its impact on BMP-4 expression, processing and degradation was investigated in prostate cancer (PC-3), osteosarcoma (HOS) and pro-monocytic (U937) human cell lines. Materials and methods. BMP-4 expression was founded to be regulated by cathepsin H using PCR array technology and confirmed by real time PCR. Immunoassays including Western blot and confocal microscopy were used to evaluate the influence of cathepsin H on BMP-4 processing. Results. In contrast to HOS, the expression of BMP-4 mRNA in U937 and PC3 cells was significantly decreased by cathepsin H. The different regulation of BMP-4 synthesis could be associated with the absence of the mature 28 kDa cathepsin H form in HOS cells, where only the intermediate 30 kDa form was observed. No co-localization of BMP-4 and cathepsin H was observed in human cell lines and the multistep processing of BMP-4 was not altered in the presence of specific cathepsin H inhibitor. Isolated cathepsin H does not cleave mature recombinant BMP-4, neither with its amino- nor its endopeptidase activity. Conclusions. Our results exclude direct proteolytic processing of BMP-4 by cathepsin H, however, they provide support for its involvement in the regulation of BMP-4 expression. katepsin H rak (medicina) proteolitični encimi morfogenetični protein 4 true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-03-18 13:52:46 2024-03-18 13:52:46 2024-03-19 03:44:39 0000-00-00 00:00:00 2011 0 0 Soavtorji: Zala Jevnikar, Bojana Mirković, Damjan Janeš, Nace Zidar, Danijel Kikelj, Janko Kos; str. 259-266, II no. 4 Vol. 45 2011 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2011-01-01 577 1318-2099 10.2478/v10019-011-0034-3 3157361 http://versita.metapress.com/content/p203104l6755v4x4/ 1 6ec5f2d0-e526-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25138 RAZ_Rojnik_Matija_2011.pdf RAZ_Rojnik_Matija_2011.pdf 1 645C9D7F13AE114496E9897DD30D5B5E e3a4f52d3c0c8dc62d98361002da282662d8db9b78d7aed20d69062b0ff562c7 9b91e76e-e526-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25139 Onkološki inštitut Ljubljana 0 0 0