20.500.12556/DiRROS-18265 Characterization of hFOB 1.19 cell line for studying Zn-based degradable metallic biomaterials In vitro testing is the first important step in the development of new biomaterials. The human fetal osteoblast cell line hFOB 1.19 is a very promising cell model; however, there are vast discrepancies in cultivation protocols, especially in the cultivation temperature and the presence of the selection reagent, geneticin (G418). We intended to use hFOB 1.19 for the testing of Zn-based degradable metallic materials. However, the sensitivity of hFOB 1.19 to zinc ions has not yet been studied. Therefore, we compared the toxicity of zinc towards hFOB 1.19 under different conditions and compared it with that of the L929 mouse fibroblast cell line. We also tested the cytotoxicity of three types of Zn-based biomaterials in two types of media. The presence of G418 used as a selection reagent decreased the sensitivity of hFOB 1.19 to Zn2+. hFOB 1.19 cell line was more sensitive to Zn2+ at elevated (restrictive) temperatures. hFOB 1.19 cell line was less sensitive to Zn2+ than L929 cell line (both as ZnCl2 and extracts of alloys). Therefore, the appropriate cultivation conditions of hFOB 1.19 during biomaterial testing should be chosen with caution. zinc degradable materials in vitro cytotoxicity testing hFOB 1.19 osteoblasts true false true MDPI Švica Angleški jezik Ni določen © 2024 by the authors Neznano 2024-02-28 07:16:19 2024-02-28 07:16:20 2024-04-04 08:53:15 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Opis vira z dne 31. 1. 2024; str. 1-15 iss. 4, [article no.] 915 Vol. 17 Feb. 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2024-02-16 620.1:669.55:617.3 1996-1944 10.3390/ma17040915 183199235 https://www.mdpi.com/1996-1944/17/4/915 1 89be53f9-d5f8-11ee-900a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24806 RAZ_Jablonska_Eva_2024.pdf RAZ_Jablonska_Eva_2024.pdf 1 07C5C7DC00B3F4DB0A0A9E1B61B9CD0E f49f3e2abbb30786f4b38ee8ce3c44db217c48307f9ace48f05cbb6988f6cd19 1c53f711-d5fb-11ee-900a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24807 Inštitut za kovinske materiale in tehnologije 0 0 0