20.500.12556/DiRROS-18112-a385887e-0fe2-397e-2343-ac8850b3ce23 Detection of apoptotic cells in tumour paraffin sections [Prikaz apoptotičnih celic v tumorskih parafinskih rezinah] Apoptosis is a distinct form of cell death characterised by specific morphological features and regulated by complex molecular mechanisms. Its deregulation is fundamental for tumour growth and progression and, moreover, anticancer therapies suppress tumour growth mainly by induction of apoptosis. Since the extent of apoptosis in a tumour may have prognostic as well as therapeutic implications, much effort has been invested in developing specificmethods that can be routinely used to detect apoptotic cells in archival formalin fixed paraffin-embedded tissue. Complex molecular pathways are involved in the regulation of apoptosis. Pro-apoptotic signals trigger activation of caspases that specifically cleave target proteins. Cleavage of proteins (caspase substrates) is responsible for morphological changes of apoptotic cells and DNA fragmentation. In the last decade, detection of apoptotic cells in formalin fixed tumour tissue sections has been based mainlyon morphology and characteristic DNA fragmentation. Recently, specific antibodies to activated caspases and cleaved target proteins (including cytokeratin 18, actin and PARP) have been produced that enable accurate detection of apoptosis in paraffin sections. Apoptoza je oblika celične smrti z značilnimi morfološkimi spremembami, ki jo uravnavajo zapleteni molekularni mehanizmi. Motnje v uravnavanju apoptoze so pomembne za rast tumorjev, hkrati pa različne metode zdravljenja tumorjev zavirajo njihovo rast pretežno s sprožanjem apoptoze tumorskih celic. Ugotavljanje apoptotske aktivnost v tumorju je lahko pomembno za napoved poteka bolezni in njenega zdravljenja, zato je pomemben razvoj metod za rutinski prikaz apoptotičnih celic v tumorskem tkivu, ki je bilo fiksirano v formalinu in vklopljeno v parafin. Pri uravnavanju apoptoze sodelujejo zapletene molekularne poti. Različni proapoptotični signali sprožijo aktivacijo kaspaz, slednje pa cepijo tarčne beljakovine. Njihova cepitev je odgovorna za morfološke spremembe apoptotičnih celic in značilno cepitev jedrne DNK. V zadnjem desetletju je prikaz apoptotičnih celic v tumorskem tkivu fiksiranem v formalinu temeljil pretežno na morfoloških značilnostih in značilni fragmentaciji DNK. V zadnjem času omogoča zanesljiv prikaz apoptotičnih celic imunohistokemična reakcija na prikaz aktiviranih kaspaz in cepljenih tarčnih beljakovin (citokeratin 18, aktin, PARP). true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-02-06 13:51:06 2024-02-06 13:51:06 2024-02-10 03:37:32 0000-00-00 00:00:00 2003 0 0 BSDOCID104069; str. 225-232 št. 4 Letn. 37 2003 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2003-01-01 616-006 1318-2099 17382873 RAZ_Pizem_Joze_2003.pdf RAZ_Pizem_Joze_2003.pdf 1 F177126867596850636A2EC604332548 d2d5a2c092a07759d8b1111a6a97c36e58aa828a12b39f70f63c79c33723f2fd 1695d7eb-c4e8-11ee-8001-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24606 Onkološki inštitut Ljubljana 0 0 0