20.500.12556/DiRROS-18106-c1ec9256-1c56-1363-e9c2-2e35111c6757 Effect of electroporation on radiosensitization with cisplatin in two cell lines with different chemo- and radiosensitivity Učinek elektroporacije s cisplatinom na radiosenzibilizacijo dveh celičnih linij z različno občutljivostjo za kemoterapevtike in obsevanje Aim. Radiosensitization with cisplatin can be enhanced by electroporation of cells and tumours. The aim of this study was to extend our previous studies ontwo carcinoma tumour models with different chemo-and radiosensitivity in order to evaluate whether this treatment is effective also on less chemo-and radiosensitive tumour cells. Materials and methods. This in vitro study was performed on carcinoma SCK and EAT-E cells. The cytotoxicity of three-modalitytreatment consisting of cisplatin, electroporation and irradiation was determined by the clonogenic assay. Results. The radiosensitizing effect of cisplatin on the two cell lines was greatly enhanced by electroporation. By this combined treatment, less chemo and radiosensitive EAT-E cells were rendered as sensitive as more chemo and radiosensitive SCK cells. Conclusion. The enhancement of cisplatin-induced radiosensitization of cells by electroporation could be beneficially used in the treatment of intrinsically less chemo- and radiosensitive tumours. Izhodišča. Z elektroporacijo celic in tumorjev lahko povečamo njihovo občutljivost za cisplatin. Namen raziskave je bil razširiti našo predhodno raziskavo na dva tumorska modela karcinoma z različno občutljivostjo za kemoterapijo in obsevanje, ter ugotoviti, ali je takšno zdravljenje učinkovitotudi pri celicah, ki so manj občutljive za kemoterapijo in obsevanje. Materiali in metode. V in vitro raziskavi smo uporabili celični liniji karcinoma SCK in EAT-E. Citotoksično delovanje kombiniranega zdravljenja s cisplatinom, elektroporacijo in obsevanjem smo določili s testomklonogenosti. Rezultati. Z elektroporacijo se je radiosenzibilizirajoči učinek cisplatina na testiranih celičnih linijah zelo povečal. Manj kemo- in radio-občutljive celice EAT-E so po kombiniranem zdravljenju postale enako občutljive kot celice SCK, ki so bolj občutljive za kemoterapijo in obsevanje.Zaključki. Povečano občutljivost celic za cisplatin po elektroporaciji lahko koristno uporabimo tudi za zdravljenje manj občutljivih celic za kemoterapijo in obsevanje. true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-02-06 11:12:09 2024-02-06 11:12:09 2024-02-10 03:36:50 0000-00-00 00:00:00 2003 0 0 BSDOCID99070; str. 101-107 št. 2 Letn. 37 June 2003 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2003-06-01 616-006 1318-2099 16558809 RAZ_Kranjc_Brezar_Simona_2003.pdf RAZ_Kranjc_Brezar_Simona_2003.pdf 1 9169C5E431788CEBB53FB72E9DDF6738 5159b060193e4c48b3f0beda3fe93547b0c2610385c9e527307cf2341e9e924b f591a031-c4cf-11ee-8001-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24598 Onkološki inštitut Ljubljana 0 0 0