20.500.12556/DiRROS-18101-a3587b01-f563-8101-0321-e4e383b02641 The urokinase plasminogen activator and its inhibitors PAI-1 nad PAI-2 in primary cutaneous melanoma [Urokinazni plazminogenski aktivator in njegova inhibitorja PAI-1 in PAI-2 pri primarnem kožnem melanomu] Background. We investigated the differences in urokinase plasminogen activator(uPA) and its inhibitors type-1 and 2 (PAI-1/2) concentrations in clinically suspected nevi, primary cutaneous melanoma and normal skin and correlations with histopathological prognostic factors of primary melanoma. Patients and methods. Fifty-one patients were enrolled. The tissue concentrations of uPA, PAI 1 and PAI2 were quantified by enzyme-linked immunosorbent assay (ELISA). Results. Mean uPA and PAI-1 concentrations in melanomas were higher than in normal surrounding skin (uPA: 1.08; vs. 0.48 ng/mgp; PAI-1: 14.07 vs. 2.07 ng/mgp; p < 0.001), uPA and PAI-1 concentrationswere higher in melanomas than in nevi, and higher in nevi than in normal surrounding skin (uPA: p > 0.05; PAI-1: p = 0.02). PAI-2 concentration was higher in normal surrounding skin than in nevi and melanomas(p > 0.05). Melanoma uPA, PAI-1 and PAI-2 concentrations correlated significantly with normal skin (r= 0.73, 0.54, 0.38 respectively). PAI 1 was significantly lower in melanomas of Breslow thickness < 0.75 mm, Clark invasion of O+I, without microscopic ulceration, without vascular invasion (p < 0.01) than in melanomas of Breslow thickness > 0.75 mm, Clark invasion > II,with ulceration and vascular invasion. Conclusions. Determination of uPA and PAI-1 can provide significant additional prognostic information for melanoma patients. Izhodišča. Ugotavljali smo razlike v koncentracijah urokinaznega plazminogenskega aktivatorja (uPA) in njegovih inhibitorjev tipa 1 in 2 (PAI-1/2) v klinično sumljivih nevusih, primarnem kožnem melanomu in zdravi koži v okolici ter korelacije s histopatološkimi napovednimi dejavniki primarnega melanoma. Bolniki in metode. Vključili smo 51 bolnikov. Tkivne koncentracije uPA, PAI-1 in PAI-2 smo merili z encimskoimunsko metodo (ELISA).Rezultati. Povprečne koncentracije uPA in PAI-1 v melanomih so bile višje kot v zdravi koži (uPA: 1,08; vs. 0,48 ng/mgp; PAI-1: 14,07 vs. 2,07 ng/mgp; p < 0,001). Koncentracije uPA in PAI1 so bile višje v melanomih kot v nevusih in višje v nevusih kot v zdravi koži (p > 0,05). Koncentracija PAI-2 je bila višja v zdravi koži kot v nevusih in melanomih (p > 0,05). Koncentracije uPA, PAI-1 in PAI-2 v melanomih so statistično značilno korelirale s koncentracijami v zdravi koži (r = 0,73; 0,54; 0,38). Koncentracije PAI-1 so bile statistično značilno nižje v melanomih debeline poBreslowu <_ 0,75 mm, invazije po Clarku O+I, z odsotno mikroskopsko ulceracijo, z odsotno vaskularno invazijo (p < 0,01) kot v melanomih debeline po Breslowu > 0,75 mm, invazije po Clarku > II, s prisotno mikroskopsko ulceracijo, s prisotno vaskularno invazijo. Zaključki. Z določitvijo uPA in PAI-1 lahko dobimo pomemben dodaten podatek o prognozi bolnikov z melanomom. true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-02-06 09:19:51 2024-02-06 09:19:51 2024-02-10 03:36:00 0000-00-00 00:00:00 2003 0 0 BSDOCID96231; str. 29-35 št. 1 Letn. 37 2003 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2003-01-01 616.5 1318-2099 16232665 RAZ_Markovic_Bozic_Jasmina_2003.pdf RAZ_Markovic_Bozic_Jasmina_2003.pdf 1 D0FA8BE6813165ADA262EE762CAC2B97 9852458cb2acdde41c9360425570c6c1d8e6646b3894791c8ef7f240e9655714 97b8a0ea-c4cb-11ee-8001-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24592 Onkološki inštitut Ljubljana 0 0 0