20.500.12556/DiRROS-18017 Morphological characteristics of young and old murine hematopoietic stem cell niches, as modeled in vitro The hematopoietic stem cell (HSC) niche undergoes detrimental changes with age. The molecular differences between young and old niches are well studied and understood; however, young and old niches have not yet been extensively characterized in terms of morphology. In the present work, a 2D stromal model of young and old HSC niches isolated from bone marrow was investigated using light and scanning electron microscopy (SEM) to characterize cell density after one, two, or three weeks of culturing, cell shape, and cell surface morphological features. Our work is aimed at identifying morphological differences between young and old niche cells that could be used to discriminate between their respective murine HSC niches. The results show several age- specific morphological characteristics. The old niches differ from the young ones in terms of lower cell proliferating capacity, increased cell size with a flattened appearance, increased number of adipocytes, and the presence of tunneling nanotubes. In addition, proliferating cell clusters are present in the young niches but not in the old niches. Together, these characteristics could be used as a relatively simple and reliable tool to discriminate between young and old murine HSC niches and as a complementary approach to imaging with specific cellular markers. bone marrow hematoopetic stem cell niche aging adipocytes scanning electron microscopy kostni mozeg niša hematopoetskih matičnih celic staranje adipociti vrstična elektronska mikroskopija true false true Hindawi Egipt Angleški jezik Slovenski jezik © 2023 Mojca Justin et al. Neznano 2024-01-26 14:24:47 2024-01-26 14:24:47 2024-04-18 11:24:14 0000-00-00 00:00:00 2023 0 0 Nasl. z nasl. zaslona; Soavtorji: Ema Rogač Randl, Veno Kononenko, Matej Hočevar, Damjana Drobne, Primož Rožman; Opis vira z dne 17. 4. 2023; str. 1-10 [Art. no.] 5541050 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-04-15 576 1932-8745 10.1155/2023/5541050 149390083 RAZ_Justin_Mojca_2023.pdf RAZ_Justin_Mojca_2023.pdf 1 E29E582B4A5E58C10BE12F9672411795 ae02e6d5e881a09a8999df71ad7ab154b4284c50c6581fcb78a5e15a70065502 53a1596f-c0cd-11ee-b0d4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24522 https://www.hindawi.com/journals/scanning/2023/5541050/ 1 474e2b1f-bc4e-11ee-b0d4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24452 Inštitut za kovinske materiale in tehnologije 0 0 0