20.500.12556/DiRROS-17991 Electroporator for in vitro cell permeabilization Elektroporator za permeabilizacijo celičnih membran in vitro The use of high voltage electric pulse technology, electroporation, in cell biology, biotechnology and medicine has attracted an enormous interest. Electroporation is a transient phenomenon that increases the permeability of cell plasma membrane. In the state of high permeability, the plasma membrane allows small and large molecules to be introduced into the cytoplasm, althoughthe cell plasma membrane represents a considerable barrier for them inits normal state. The effectiveness of electroporation depends on many parameters that can be divided into the parameters of the electric field and the parameters that define the state of cells and their surrounding i.e, temperature, osmotic pressure, etc. In this article, we present a prototype electroporator GT-1 for in vitro electropermeabilization that we have developed. Our electroporator offers a vast flexibility of parameters and can generate high and low voltage pulses, of which the latter ones are used for electrophoretic transfer of charged molecules through permeabilized cell plasma membrane. electroporation instrumentation methods cell membrane permeabilization inštrumentacijske metode elektroporacije permeabilizacija celične membrane true false true Slovenian Medical Association - Slovenian Association of Radiology, Nuclear Medicine Society, Slovenian Society far Radiotherapy and Oncology, and Slovenian Cancer Society Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-01-25 13:43:13 2024-01-25 13:43:14 2024-01-26 03:50:48 0000-00-00 00:00:00 2001 0 0 str. 203-207 no. 3 Vol. 35 september 2001 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2001-09-01 61 1318-2099 2436948 RAZ_Puc_Marko_2001.pdf RAZ_Puc_Marko_2001.pdf 1 406EEFD97FC18E8A56C01A60E9A561E0 4d47632571a9672696fb785d78f20f24c94103f042c79ca8c5c8c48c88829e4d a684a256-bb7f-11ee-b0d4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24410 Onkološki inštitut Ljubljana 0 0 0