20.500.12556/DiRROS-17929 Reorganization of microtubules in V-79 cells after treatment with cytohalasin B Razporejanje mikrotubulov v celicah V-79 po delovanju citohalazina B Background. The aim of this work was to study the configuration of the microtubules in the cytochalasin B treated V-79 cells in connection to the cell shape and to see whether there are any similarities to the phenomena taking place in phospholipid vesicles. Subjects and methods. An experiment wasperformed where cytochalasin B was added to the V-79 cells (lung fibroblasts of Chinese hamster). Results. The cell shape changed from an elongated one into the shape with a profile resembling the Greek letter phi. The microtubules were found to be organized into a rod within the symmetry axis of the cell. Conclusion. As similar shapes were previously observed also in the phospholipid vesicles with entrapped microtubule rods, our results support the hypothesis that similar physical mechanisms may pertain in simple systems as well as in living cells. Izhodišča. V tej raziskavi smo proučevali razporeditev mikrotubulov celicah V-79 po delovanju citohalazina B. Poleg njihove oblike smo poskušali ugotoviti, ali je ta pojav v čemer koli podoben dogajanjem v fosfolipidnih mehurčkih. Material in metode. Opravili smo preiskus s citohalazinom B, ki smoga dodali celicam V 79 (pljučni fibroblasti kitajskega hrčka). Rezultati. Oblika celice se je spremenila iz podolgovate v obliko, katere profil je podoben grški črki fi. Mikrotubuli so se uredili v palico v simetrijski osi celice. Zaključek. Ker so podobne oblike pred tem opazili tudi pri fosfolipidnih mehurčkih, ki so vsebovali paličasto strukturo iz mikrotubulov, prikazani rezultati podpirajo hipotezo, da so podobni fizikalni mehanizmi prisotni v preprostih sistemih kot tudi v živih celicah. true false true Slovenian Medical Association - Slovenian Association of Radiology, Nuclear Medicine Society, Slovenian Society far Radiotherapy and Oncology, and Slovenian Cancer Society Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-01-22 13:52:19 2024-01-22 13:52:19 2024-01-23 03:41:25 0000-00-00 00:00:00 1999 0 0 BSDOCID49540; str. 297-301 št. 4 Letn. 33 december 1999 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 1999-12-01 616-006 1318-2099 11071961 RAZ_Iglic_Ales_1999.pdf RAZ_Iglic_Ales_1999.pdf 1 BFC127C24FE50D3517DDC47021BD34B2 083ba6c9cf96639576b1c4ca07081d7132cef028962b6271cffef73a27c58737 562eab69-b925-11ee-b0d4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24316 Onkološki inštitut Ljubljana 0 0 0