20.500.12556/DiRROS-17912 Description, identification, and growth of Tuber borchii Vittad. mycorrhized Pinus sylvestris L. seedlings on different lime contents Tuber borchii forms ectomycorrhiza with oaks, hazel, and pines, including Pinus sylvestris. However, its ectomycorrhiza morphotype with P. sylvestris was not comprehensively described so far, and molecular analyses are missing despite a high danger of misidentification of T. borchii ectomycorrhiza with other closely related and less valuable truffle species. We described for the first time the morphology and anatomy of T. borchii-P. sylvestris ectomycorrhiza using differential interference contrast technique and semi-thin sections in combination with molecular confirmation of identity. Color of ectomycorrhiza is reddish to dark brown, and morphotypes are unevenly but densely covered by warts-bearing pin-like cystidia. All layers of the hyphal mantle are pseudoparenchymatous with outer mantle layer formed of epidermoid cells. T. borchii ectomycorrhiza was identified by a molecular comparison with fruitbodies used for inoculation and its respective ectomycorrhizae. T. borchii has a wide ecological amplitude. To get a better insight in mycorrhization requirements, we investigated growth of P. sylvestris and its ectomycorrhiza infection rate with T. borchii in substrate with different lime content. The mycorrhization of P. sylvestris with T. borchii in the mycorrhization substrate and cultivation in greenhouse conditions was successful, with colonization of P. sylvestris varying between 36.5 and 48.1%. There was no significant correlation of mycorrhization to applied lime contents, and consequently to pH in substrate, while the increased levels of lime improved growth of the P. sylvestris seedlings. root anatomy ectomycorrhiza Limestone content molecular identification Pinus sylvestris Tuber borchii anatomija korenin ektomikoriza vsebnost karbonata v substratu molekularna identifikacija bor Pinus sylvestris gomoljika tartuf Tuber borchii true false true Angleški jezik Slovenski jezik Neznano 2024-01-22 11:22:51 2024-01-22 11:22:52 2024-05-08 14:51:39 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Opis vira z dne 22. 1. 2024; Avtorja iz Slovenije: Tanja Mrak, Tine Grebenc; str. 85-94 iss. Vol. 34 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2024-01-01 630*16 1432-1890 10.1007/s00572-023-01135-3 181909251 https://link.springer.com/article/10.1007/s00572-023-01135-3 0 34dd8993-b910-11ee-b0d4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24293 RAZ_Mrak_Tanja_2024.pdf RAZ_Mrak_Tanja_2024.pdf 1 ADBBEFF7D4E04570E64077A7421D409E b7fd6ab27d8b8b48fefb71c38ea6cfb9e45a55038fb6c7635757489a7bec82a6 4f46d695-b910-11ee-b0d4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24294 Gozdarski inštitut Slovenije 0 0 0