20.500.12556/DiRROS-17878 Genetic polymorphisms of xenobiotic metabolizing enzymes in human colorectal cancer Genetski polimorfizem encimov, ki presnavljajo ksenobiotike pri kolorektalnem raku It was proposed that both hereaditary and environmental factors contribute to the development of colorectal cancer (CRC). Carcinogenic polycyclic aromatic hydrocarbons (PAHs) from food or tobacco smoke can form DNA adducts and thus initiate carcinogenesis after metabolic activation via cytochrome P4501A1 (CYP1A1). Intermediate metabolites are detoxified by conjugation with glutathione S-transferases. Our aim was to look for inherited metabolic suceptibility to CRC. We used PCR-based genotyping approach to determine the frequencies of polymorphic alleles of two cytochromes P450 (CYP2D6 and CYP1A1)and two glutathione S-transferases (GSTM1 and GSTT1) in DNA samples from 31 sporadic, 25 familial CRC cases and 73 healthy controls. The difference in frequencies of poor metabolisers due to CYP2D6 gene polymorphismwas close to the limit of statistical significance between sporadiCRC and healthy control group (lambda 2=5.52, m=2, p=0.06) despite the small sample size. The frequencies of either CYP1A1 MspI, GST M1 or GST T1 genotypes were not significantly different in both CRC cases and in controls. Although our study suggests some difference in metabolic susceptibility between sporadic and familial CRC, further studies are needed to investigate the combined effect of polymorphic genes involved in carcinogen metabolism in a larger group of patients with defined exposure to dietary carcinogens and smoking. true false false Slovenian Medical Society - Section of Radiology ; Croatian Medical Association - Croatian Society of Radiology Ljubljana Angleški jezik Slovenski jezik by Authors Neznano 2024-01-19 10:44:08 2024-01-19 10:44:09 2024-01-20 03:46:19 0000-00-00 00:00:00 1998 0 0 Life sciences '97 and 2nd Slovenian-Croatian meeting on Molecular oncology today; 1997 Oct 16-19; Gozd Martuljek; BSDOCID37103; str. 35-39 št. 1 Let. 32 1998 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 1998-01-01 616-006 1318-2099 7555289 RAZ_Dolzan_Vita_1998.pdf RAZ_Dolzan_Vita_1998.pdf 1 8EABA9DD00518D607F1B1C5AE8C73EF7 b1efd16061514b51cc110023a43c6b3dc9d31eb740d9bdfaa8c88d833b537968 809d389f-b6af-11ee-b0d4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=24239 Onkološki inštitut Ljubljana 0 0 0