20.500.12556/DiRROS-16723-0d415d85-90b9-5532-10fe-a76a0105e156 Epidemiology of Flavescence dorée and hazelnut decline in Slovenia geographical distribution and genetic diversity of the associated 16SrV phytoplasmas Epidemiology of Flavescence dorée and hazelnut decline in Slovenia: geographical distribution and genetic diversity of the associated 16SrV phytoplasmas Flavescence dorée (FD) phytoplasma from 16SrV-C and -D subgroups cause severe damage to grapevines throughout Europe. This phytoplasma is transmitted from grapevine to grapevine by the sap-sucking leafhopper Scaphoideus titanus. European black alder and clematis serve as perennial plant reservoirs for 16SrV-C phytoplasma strains, and their host range has recently been extended to hazelnuts. In Slovenia, hazelnut orchards are declining due to 16SrV phytoplasma infections, where large populations of the non-autochthonous leafhopper Orientus ishidae have been observed. To better characterise the phytoplasma-induced decline of hazelnut and possible transmission fluxes between these orchards and grapevine, genetic diversity of 16SrV phytoplasmas in grapevine, hazelnut and leafhoppers was monitored from 2017 to 2022. The nucleotide sequence analysis was based on the map gene. The most prevalent map genotype in grapevine in all wine-growing regions of Slovenia was M54, which accounted for 84 % of the 176 grapevines tested. Besides M54, other epidemic genotypes with lower frequency were M38 (6 %), M51 (3 %), M50 (2 %) and M122 (1 %). M38, M50 and M122 were also detected in infected cultivated hazelnuts and in specimens of O. ishidae leafhopper caught in declining hazelnut orchards. It suggests that this polyphagous vector could be responsible for phytoplasma infection in hazelnut orchards and possibly for some phytoplasma exchanges between hazelnuts and grapevine. We hereby describe new genotypes: M158 in grapevine as well as four never reported genotypes M159 to M162 in hazelnut. Of these four genotypes in hazelnut, one (M160) was also detected in O. ishidae. Analysis of additional genes of the new genotypes allowed us to assign them to the VmpA-III cluster, which corresponds to the 16SrV-C strains previously shown to be compatible with S. titanus transmission. phytoplasmas haselnuts grapevine Flavescence dorée epidemiology plant disease fitoplazme leska trta zlata trsna rumenica bolezni vinske trte rastlinske bolezni Vitis vinifera Corylus avellana true false true Angleški jezik Latinski jezik Neznano 2023-07-26 15:37:50 2023-07-26 15:37:52 2024-05-23 11:48:38 0000-00-00 00:00:00 2023 0 0 Soavtorji: Gabrijel Seljak, Tjaša Jakomin, Jakob Brodarič, Ana Vučurović, Sandra Pedemay, Pascal Salar, Sylvie Malembic-Maher, Xavier Foissac and Nataša Mehle; Nasl. z nasl. zaslona; Opis vira z dne 18. 7. 2023; str. 1-12 [art.] 1217425 Vol. 14 2023 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2023-07-04 632 1664-462X 159155203 https://doi.org/10.3389/fpls.2023.1217425 1 cb3762ab-2ba8-11ee-8cc8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=22717 https://www.frontiersin.org/articles/10.3389/fpls.2023.1217425/full#supplementary-material 1 90d03c30-edb7-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25348 https://doi.org/10.3389/fpls.2023.1261658 1 a7f72759-edb7-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25350 RAZ_Kogej_Zwitter_Zala_2023.pdf RAZ_Kogej_Zwitter_Zala_2023.pdf 1 2C46E570BF605DE1B5139DF67837A145 1b05341a986bc3068812936e42c6dabbf2bb0f886e9ddb218a1b188269f8f3b6 035e0396-edb8-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25352 RAZ_Kogej_Zwitter_Zala_2023.pdf RAZ_Kogej_Zwitter_Zala_2023.pdf 1 1E20566584375F2BC7FAC239F9D72FCA 8fd62d8df0ea1a8ed0768e5ba24134aa33f6f9540b85c9f396423fc85c61f247 2555524b-edb8-11ee-9dd4-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=25353 Nacionalni inštitut za biologijo 0 0 0