20.500.12556/DiRROS-16440 Chloroplast redox state changes mark cell-to-cell signaling in the hypersensitive response Bisphenol A (BPA) is one of the most commonly used substances in the manufacture ofvarious everyday products. Growing concerns about its hazardous properties, including endocrinedisruption and genotoxicity, have led to its gradual replacement by presumably safer analogues inmanufacturing plastics. The widespread use of BPA and, more recently, its analogues has increasedtheir residues in the environment. However, our knowledge of their toxicological profiles is limitedand their combined effects are unknown. In the present study, we investigated the toxic effectscaused by single bisphenols and by the combined exposure of BPA and its two analogues, BPAP andBPC, after short (24-h) and prolonged (96-h) exposure in HepG2 spheroids. The results showed thatBPA did not reduce cell viability in HepG2 spheroids after 24-h exposure. In contrast, BPAP andBPC affected cell viability in HepG2 spheroids. Both binary mixtures (BPA/BPAP and BPA/BPC)decreased cell viability in a dose-dependent manner, but the significant difference was only observedfor the combination of BPA/BPC (both at 40μM). After 96-h exposure, none of the BPs studiedaffected cell viability in HepG2 spheroids. Only the combination of BPA/BPAP decreased cellviability in a dose-dependent manner that was significant for the combination of 4μM BPA and 4μMBPAP. None of the BPs and their binary mixtures studied affected the surface area and growth ofspheroids as measured by planimetry. In addition, all BPs and their binary mixtures studied triggeredoxidative stress, as measured by the production of reactive oxygen species and malondialdehyde,at both exposure times. Overall, the results suggest that it is important to study the effects of BPsas single compounds. It is even more important to study the effects of combined exposures, as thecombined effects may differ from those induced by single compounds. chloroplast redox state hypersensitive response (HR)-conferred resistance immune signaling live cell imaging Solanum tuberosum (potato) spatiotemporal analysis stromules virus resistance redoks stanje kloroplastov krompir preobčutljivostni odgovor rastlinska imunska signalizacija true false true Angleški jezik Slovenski jezik Neznano 2023-04-13 08:39:23 2023-04-13 08:39:23 2024-05-24 03:40:29 0000-00-00 00:00:00 2023 0 0 Nasl. z nasl. zaslona; Opis vira z dne 19. 9. 2022; str. 548-562 iss. 2 Vol. 237 2023 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2023-01-01 57 1469-8137 10.1111/nph.18425 121824771 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.18425 1 72cd72ed-d9bd-11ed-b359-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=22131 New_Phytologist_-_2022_-_Lukan_-_Chloroplast_redox_state_changes_mark_cell‐to‐cell_signaling_in_the_hypersensitive_response.pdf New_Phytologist_-_2022_-_Lukan_-_Chloroplast_redox_state_changes_mark_cell‐to‐cell_signaling_in_the_hypersensitive_response.pdf 1 613AF4FFF2AA9C78F82C598C1293205A 06fe5a210ece2c3d121a613cab75e384851417379187fb0283f0ff9edb50b038 085f408d-d9bf-11ed-b359-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=22133 Nacionalni inštitut za biologijo 0 0 0