20.500.12556/DiRROS-16089 Multiscale femoral neck imaging and multimodal trabeculae quality characterization in an osteoporotic bone sample Although multiple structural, mechanical, and molecular factors are definitely involved in osteoporosis, the assessment of subregional bone mineral density remains the most commonly used diagnostic index. In this study, we characterized bone quality in the femoral neck of one osteoporotic patients as compared to an age-matched control subject, and so used a multiscale and multimodal approach including X-ray computed microtomography at different spatial resolutions (pixel size: 51.0, 4.95 and 0.9 µm), microindentation and Fourier transform infrared spectroscopy. Our results showed abnormalities in the osteocytes lacunae volume (358.08 ± 165.00 for the osteoporotic sample vs. 287.10 ± 160.00 for the control), whereas a statistical difference was found neither for shape nor for density. The osteoporotic femoral head and great trochanter reported reduced elastic modulus (Es) and hardness (H) compared to the control reference (−48% (p < 0.0001) and −34% (p < 0.0001), respectively for Es and H in the femoral head and −29% (p < 0.01) and −22% (p < 0.05), respectively for Es and H in the great trochanter), whereas the corresponding values in the femoral neck were in the same range. The spectral analysis could distinguish neither subregional differences in the osteoporotic sample nor between the osteoporotic and healthy samples. Although, infrared spectroscopic measurements were comparable among subregions, and so regardless of the bone osteoporotic status, the trabecular mechanical properties were comparable only in the femoral neck. These results illustrate that bone remodeling in osteoporosis is a non-uniform process with different rates in different bone anatomical regions, hence showing the interest of a clear analysis of the bone microarchitecture in the case of patients’ osteoporotic evaluation synchrotron X-ray microtomography bone trabecular struicture osteons osteoporosis phase-contrast imaging open access sinhrotronska rentgenska mikrotomografija kosti trabekularna struktura osteoni osteoporoza slikanje s faznim kontrastom odprti dostop true false true Molecular Diversity Preservation International Angleški jezik Slovenski jezik © 2022 by the authors. Licensee MDPI, Basel, Switzerland Neznano 2023-01-20 07:22:58 2023-04-28 08:14:00 2023-04-29 03:36:37 0000-00-00 00:00:00 2022 0 0 Nasl. z nasl. zaslona; Opis vira z dne 5. 1. 2023; Št. članka 8048; str. 1-24 iss. 12 Vol. 15 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-11-14 61 1996-1944 10.3390/ma15228048 136456195 https://www.mdpi.com/1996-1944/15/22/8048 1 e4535a13-988a-11ed-b359-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=21399 RAZ_Soldati_Enrico_2022.pdf RAZ_Soldati_Enrico_2022.pdf 1 A2B9D3AA82FA4C34C9B2105DCC71A10D f281f189321278967e0e39637d38d9621e85ec5054ebb9bcd4fcb27c8546492c 66c4af48-988c-11ed-b359-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=21400 Zavod za gradbeništvo Slovenije 0 0 0