20.500.12556/DiRROS-15911 Impact of 10-day bed rest on serum levels of irisin and markers of musculoskeletal metabolism The bed rest (BR) is a ground-based model to simulate microgravity mimicking skeletal-muscle alterations as in spaceflight. Molecular coupling between bone and muscle might be involved in physiological and pathological conditions. Thus, the new myokine irisin and bone-muscle turnover markers have been studied during and after 10 days of BR. Ten young male individuals were subjected to 10 days of horizontal BR. Serum concentrations of irisin, myostatin, sclerostin, and haptoglobin were assessed, and muscle tissue gene expression on vastus lateralis biopsies was determined. During 10-days BR, we observed no significant fluctuation levels of irisin, myostatin, and sclerostin. Two days after BR (R+2), irisin serum levels significantly decreased while myostatin, sclerostin, and haptoglobin were significantly increased compared with BR0. Gene expression of myokines, inflammatory molecules, transcription factors, and markers of muscle atrophy and senescence on muscle biopsies were not altered, suggesting that muscle metabolism of young, healthy subjects is able to adapt to the hypomobility condition during 10-day BR. However, when subjects were divided according to irisin serum levels at BR9, muscle ring finger-1 mRNA expression was significantly lower in subjects with higher irisin serum levels, suggesting that this myokine may prevent the triggering of muscle atrophy. Moreover, the negative correlation between p21 mRNA and irisin at BR9 indicated a possible inhibitory effect of the myokine on the senescence marker. In conclusion, irisin could be a prognostic marker of hypomobility-induced muscle atrophy, and its serum levels could protect against muscle deterioration by preventing and/or delaying the expression of atrophy and senescence cellular markers. gibalna neaktivnost bed rest celična senesenca haptoglobin irisin mišična atrofija miostatin sklerostin physical inactivity cell senescence muscle atrophy myostatin sclerostin true false false Angleški jezik Slovenski jezik © 2022 The Authors. Neznano 2022-12-13 16:21:19 2022-12-13 16:21:21 2022-12-14 03:37:27 0000-00-00 00:00:00 2023 0 0 Nasl. z nasl. zaslona; Soavtorji: Giuseppina Storlino, Manuela Dicarlo, Roberta Zerlotin, Patrizia Pignataro, Lorenzo Sanesi, Marco Narici, Rado Pišot, Bostjan Simunič, Graziana Colaianni, Maria Grano, Silvia Colucci; Opis vira z dne 13. 12. 2022; Članek št. e22668; str. 1-13 iss. 1 Vol. 37 Jan. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 2022-11-11 2022-12-07 612.74:796.01 1530-6860 10.1096/fj.202201005RR 133716995 https://doi.org/10.1096/fj.202201005RR 1 6b137430-7af1-11ed-8925-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=21052 ZRSKP_SimunicB_133716995.pdf ZRSKP_SimunicB_133716995.pdf 1 68F26ED76294F64114ECA1596E81C10F 8502a355be147ffb2291fa94de64995c2abc7086a54ebfad4a3e1e7bb3494a4d ac2f3faa-7af1-11ed-8925-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=21053 Znanstveno-raziskovalno središče Koper / Centro di Ricerche Scientifiche Capodistria 0 0 0