20.500.12556/DiRROS-15562 Plants play a crucial role in the development of soil fungal communities in the remediated substrate after EDTA washing of metal-contaminated soils In this study, we investigated the importance of plant cover for secondary succession and soil fungal community development in remediated substrates after EDTA washing of metal-contaminated soils. The abundance of the total fungal community, determined by ITS fungal marker genes (Internal Transcribed Spacer region), and root colonisation by arbuscular mycorrhizal (AM) fungi were monitored in two types of soil material (calcareous and acidic) sown with perennial ryegrass (Lolium perenne L.) and without plant cover (bulk soil). Four months after the start of the experiment, the abundance of ITS genes in the soil clearly showed that the presence of plants was the main factor affecting the total fungal community, which increased in the rhizosphere soil in most treatments, while it remained at a low level in the bulk soil (without plants). Interestingly, the addition of environmental inoculum, i.e., rhizosphere soil from a semi-natural meadow, did not have a positive effect on the abundance of the total fungal community. While fungal ITS genes were detected in soils at the end of the first growing season, arbuscular mycorrhizal (AM) structures were scarce in Lolium roots in all treatments throughout the first season. However, in the second season, more than a year after the start of the experiment, AM fungal colonisation was detected in Lolium roots in virtually all treatments, with the frequency of colonised root length ranging from 30% to >75% in some treatments, the latter also in remediated soil. This study demonstrates the importance of plants and rhizosphere in the development and secondary succession of fungal communities in soil, which has important implications for the revitalisation of remediated soils and regenerative agriculture. heavy metals arbuscular mycorrhiza remediation revitalisation secondary succession biodiversity qPCR toxic metals pollution težke kovine mikoriza remediacija revitalizacija sekundarno sukcesija biodiverziteta qPCR onesnaženje s strupenimi kovinami true false true Angleški jezik Slovenski jezik Neznano 2022-09-19 13:54:43 2022-09-19 13:54:45 2022-09-20 03:35:26 0000-00-00 00:00:00 2022 0 0 Nasl. z nasl. zaslona; Opis vira z dne 19. 9. 2022; 11 str. art. :978850 Vol. 10 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-01-01 630* 2296-665X 10.3389/fenvs.2022.978850 121915395 https://www.frontiersin.org/articles/10.3389/fenvs.2022.978850/full?&utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Environmental_Science&id=978850 1 1a030a8b-3801-11ed-bea5-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=20585 RAZ_Macek_Irena_2022.pdf RAZ_Macek_Irena_2022.pdf 1 C46A3A818A2AA6ABA957AD07671C22BF 62ece2da1eb1d906e18fa6c7d14fa5c5c39444b03b9cd6244963301bd98479d6 2fe81d94-3801-11ed-bea5-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=20586 Gozdarski inštitut Slovenije 0 0 0