20.500.12556/DiRROS-15560 Gene electrotransfer of proinflammatory chemokines CCL5 and CCL17 as a novel approach of modifying cytokine expression profile in the tumor microenvironment The effectiveness of immunotherapy highly correlates with the degree and the type of infiltrated immune cells in the tumor tissue. Treatments based on modifying the immune cell infiltrate of the tumor microenvironment are thus gaining momentum. Therefore, the aim of our study was to investigate the effects of gene therapy with two proinflammatory chemokines CCL5 and CCL17 on inflammatory cytokine expression profile and immune cell infiltrate in two murine breast tumor models, 4T1 and E0771, and two murine colon tumor models, CT26 and MC38. In vitro, lipofection of plasmid DNA encoding CCL5 or CCL17 resulted in changes in the cytokine expression profile similar to control plasmid DNA, implying that the main driver of these changes was the entry of foreign DNA into the cell%s cytosol. In vivo, gene electrotransfer resulted in high expression levels of both Ccl5 and Ccl17 transgenes in the 4T1 and CT26 tumor models. Besides a minor increase in the survival of the treated mice, the therapy also resulted in increased expression of Cxcl9 and Ifn%, potent activators of the immune system, in CT26 tumors. However, this was not recapitulated in changes of TME, implying that a further refinement of the dosing schedule is needed. chemokines cytokine expression gene electrotransfer CCL5 kemokini citokinsko izražanje genski elektroprenos CCL5 true false true Elsevier Angleški jezik Slovenski jezik by Authors Neznano 2022-09-17 03:40:11 2022-09-19 10:09:26 2022-09-30 15:11:57 0000-00-00 00:00:00 2021 0 0 str. 1-107795-13-107795 Vol. 140 2021 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2021-03-09 602.6/.7 1567-5394 10.1016/j.bioelechem.2021.107795 55465219 RAZ_Bozic_Tim_2021.pdf RAZ_Bozic_Tim_2021.pdf 1 A0CA585BC889F595C5E4FA30953968EC 87360f21ebce14e1f7a7497aa84d527fe592db88cd3c094149a785783f6f4c58 c1dfe2c2-37e1-11ed-bea5-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=20582 Onkološki inštitut Ljubljana 0 0 0