20.500.12556/DiRROS-15437 The Carniolan honeybee from Slovenia a complete and annotated mitochondrial genome with comparisons to closely related Apis mellifera subspecies The complete mitochondrial genome of the Carniolan honeybee (Apis mellifera carnica) from Slovenia, a homeland of this subspecies, was acquired in two contigs from WGS data and annotated. The newly obtained mitochondrial genome is a circular closed loop of 16,447 bp. It comprises 37 genes (13 protein coding genes, 22 tRNA genes, and 2 rRNA genes) and an AT-rich control region. The order of the tRNA genes resembles the order characteristic of A. mellifera. The mitogenomic sequence of A. m. carnica from Slovenia contains 44 uniquely coded sites in comparison to the closely related subspecies A. m. ligustica and to A. m. carnica from Austria. Furthermore, 24 differences were recognised in comparison between A. m. carnica and A. m. ligustica subspecies. Among them, there are three SNPs that affect translation in the nd2, nd4, and cox2 genes, respectively. The phylogenetic placement of A. m. carnica from Slovenia within C lineage deviates from the expected position and changes the perspective on relationship between C and O lineages. The results of this study represent a valuable addition to the information available in the phylogenomic studies of A. mellifera—a pollinator species of worldwide importance. Such genomic information is essential for this local subspecies’ conservation and preservation as well as its breeding and selection. western honeybee Apis mellifera carnica mitochondrial genome phylogeny C lineage annotation whole genome sequencing protection conservation čebelarstvo čebele mitohondrijski genom filogenija C linija sekvenciranje celotnega genoma zaščita ohranjanje true true false Angleški jezik Slovenski jezik Delo ni kategorizirano 2022-09-05 13:31:31 2022-09-06 09:52:41 2022-09-07 03:38:01 0000-00-00 00:00:00 0 0 0 Soavtorji: Andraž Marinč, Polonca Ferk, Brane Leskošek, Mai-Britt Mosbech, Ignas Bunikis, Olga Vinnere Pettersson, Lucile Soler, Janez Prešern; Nasl. z nasl. zaslona; Opis vira z dne 25. 4. 2022; str. 1-20 iss. 5 Vol. 13 May 2022 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 2022-05-01 638 2075-4450 10.3390/insects13050403 105816323 Moskric-The_Carniolan_Honeybee_from_Slovenia-Insects.pdf Moskric-The_Carniolan_Honeybee_from_Slovenia-Insects.pdf 1 78DEBB2DF0C23104862881567457EF49 63e58682bec903edee7f32e6116d536561c6912eb2e20bfb970f27a76de1fbb3 93cab596-2cfd-11ed-bea5-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=20419 Kmetijski inštitut Slovenije 0 0 0