20.500.12556/DiRROS-15139 Geographical and temporal diversity of ‘Candidatus Phytoplasma solani' in wine-growing regions in Slovenia and Austria As the causal agent of the grapevine yellows disease Bois noir, ‘Candidatus Phytoplasma solani’ has a major economic impact on grapevines. To improve the control of Bois noir, it is critical to understand the very complex epidemiological cycles that involve the multiple “Ca. P. solani” host plants and insect vectors, of which Hyalesthes obsoletus is the most important. In the present study, multiple genotyping of the tuf, secY, stamp, and vmp1 genes was performed. This involved archived grapevine samples that were collected during an official survey of grapevine yellows throughout the wine-growing regions of Slovenia (from 2003 to 2016), plus samples from Austrian grapevines, stinging nettle, field bindweed, and insect samples (collected from 2012 to 2019). The data show that the tuf-b2 type of the tuf gene has been present in eastern Slovenia since at least 2003. The hypotheses that the occurrence of the haplotypes varies due to the geographical position of Slovenia on the Italian–Slovenian Karst divide and that the haplotypes are similar between Slovenian and Austrian Styria were confirmed. The data also show haplotype changes for host plants and H. obsoletus associated with ‘Ca. P. solani,’ which might be linked to new epidemiological cycles of this phytoplasma that involve not just new plant sources and new insect vectors, but also climate and land-use changes. Bois noir genotyping ‘Ca. P. solani’ tuf gene secY survey tuf-b2 stamp true false true Angleški jezik Ni določen Neznano 2022-06-10 12:04:49 2022-06-10 12:04:50 2024-07-17 03:59:10 0000-00-00 00:00:00 2022 0 0 str. 1-12 Vol. 13 Mai 2022 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2022-05-20 632 1664-462X 10.3389/fpls.2022.889675 109003011 RAZ_Mehle_Natasa_i2022.pdf RAZ_Mehle_Natasa_i2022.pdf 1 6AD8B9045B9D3E601DF3A6622D8214E0 e09a27629cfbba228cb1b2ca74a3144e70a6a9c3cbd97623cda7180c29c4fd42 ebfcac83-17b6-11ed-b6b8-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=19810 https://doi.org/10.3389/fpls.2022.889675 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=19809 RAZ_Mehle_Natasa_2022.pdf RAZ_Mehle_Natasa_2022.pdf 1 E5461B013DE9334BFC375965CE25EC9C 95a928d425ffddb00c2972bc798fdc89869bfda615a0abdf7be2e8a6121d370c b4ba63be-437b-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=26736 Nacionalni inštitut za biologijo 0 0 0