20.500.12556/DiRROS-13474 Phytoplasmas associated with declining of hazelnut (Corylus avellana) in Slovenia Hazelnut (Corylus avellana) is cultivated on 118 ha and ranks eighth in Slovenian fruit growing production, representing 2.8% of the total area of fruit plantations in the country. However, decline of some of the trees appeared in 2012 in two plantations located in eastern Slovenia. Together these orchards cover 5 ha, with around 1600 trees planted 12 to 15 years ago. By October 2018, ~12% of these trees had died, and an additional 12% showed decay symptoms. The dead and dying trees were scattered throughout both orchards, with no apparent pattern. The most affected cultivar was ‘Istrska dolgoplodna leska’. Using molecular diagnostic methods, we showed infection of symptomatic trees with three unrelated phytoplasmas: ‘Candidatus Phytoplasma fragariae’, of the 16SrXII-E phytoplasma subgroup, and phytoplasma of the 16SrV and 16SrIX groups. In 2018, the presence of ‘Ca. P. fragariae’ and/or phytoplasma of 16SrV group were confirmed in decayed hazelnut trees in eastern, north-eastern, central, south-eastern and western Slovenia. ‘Ca. P. fragariae’ has also been detected in a forest in south-western Slovenia, for Acer campestre, Carpinus betulus, Crataegus laevigata, Fraxinus ornus and Quercus petraea. All infected forest trees showed unusual dense proliferation of sprouts from roots and/or trunks. Molecular characterisations of partial 16S rRNA, secY, map and ribosomal protein genetic locus of hazelnut 16SrV phytoplasma isolates show that they are identical to isolates that can cause grapevine flavescence dorée disease. Here, the results of our recent study and the open questions on this burning issue for hazelnut production are presented. Corylus avellana ʼCandidatus Phytoplasma fragariaeʼ 16SrV phytoplasma group 16SrIX phytoplasma group decline true false true Angleški jezik Angleški jezik Delo ni kategorizirano 2020-12-21 08:06:49 2024-07-24 09:05:04 2024-07-25 04:14:44 0000-00-00 00:00:00 2019 0 0 str. 1117-1132 iss. 4 Vol. 155 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2019-09-03 0929-1873 577 10.1007/s10658-019-01839-3 5156175 336759 http://dx.doi.org/10.1007/s10658-019-01839-3 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=16456 RAZ_Mehle_Natasa_2019.pdf RAZ_Mehle_Natasa_2019.pdf 1 567D117B5133B10BED47CE37134ACED2 1bfbf2c75a5d71efc9d078cfcc8f760c9e216e7c59a7847718b54f4567c9b9e7 8c166949-497a-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27246 Nacionalni inštitut za biologijo 0 0 0